| Autor: |
Karumban KS; Department of Chemistry, Indian Institute of Technology, Kandi, Sangareddy, Hyderabad 502284, Telangana, India. smaji@chy.iith.ac.in., Muley A; Department of Chemistry, Indian Institute of Technology, Kandi, Sangareddy, Hyderabad 502284, Telangana, India. smaji@chy.iith.ac.in., Raut R; Department of Biotechnology, Indian Institute of Technology, Kandi, Sangareddy, Hyderabad 502284, Telangana, India. ashishmisra@bt.iith.ac.in., Gupta P; Department of Biotechnology, Indian Institute of Technology, Kandi, Sangareddy, Hyderabad 502284, Telangana, India. ashishmisra@bt.iith.ac.in., Giri B; Department of Chemistry, Indian Institute of Technology, Kandi, Sangareddy, Hyderabad 502284, Telangana, India. smaji@chy.iith.ac.in., Kumbhakar S; Department of Chemistry, Indian Institute of Technology, Kandi, Sangareddy, Hyderabad 502284, Telangana, India. smaji@chy.iith.ac.in., Misra A; Department of Biotechnology, Indian Institute of Technology, Kandi, Sangareddy, Hyderabad 502284, Telangana, India. ashishmisra@bt.iith.ac.in., Maji S; Department of Chemistry, Indian Institute of Technology, Kandi, Sangareddy, Hyderabad 502284, Telangana, India. smaji@chy.iith.ac.in. |
| Abstrakt: |
Mononuclear Co(II) complexes [Co II (L)Cl 2 ]; 1, [Co II (L)(bpy)Cl]PF 6 ; 2, [Co II (L)(phen)Cl]PF 6 ; 3 and [Co II (L)(pic)Cl]; 4, (where L = N , N -bis(pyridin-2-ylmethyl)aniline, bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline, pic = picolinic acid) were systematically synthesized and characterized by different analytical and spectroscopic methods. All the complexes were structurally identified by single-crystal X-ray diffraction analysis. Penta-coordinated complex 1 adopted a distorted trigonal bipyramidal geometry, whereas hexacoordinated complexes 2-4 have distorted octahedral geometry. The interactions of salmon sperm DNA (ss-DNA) with our synthesized complexes 1-4 were investigated by absorbance and fluorescence spectroscopy. All the complexes are very susceptible to DNA binding and exhibited binding affinities ( K b ) in the order of ∼10 4 M -1 , indicating their strong interaction with ss-DNA. The Stern-Volmer constant ( K sv ) ranged from 0.46 ± 0.01 × 10 4 to 1.08 ± 0.04 × 10 4 M -1 , suggesting weak or moderate binding with DNA. Agarose gel electrophoresis revealed the DNA cleavage activity in vitro for 2-4, which could efficiently cleave the supercoiled plasmid DNA without any external agents; however, with the addition of H 2 O 2 , the cleavage property was enhanced. Live-cell imaging and other biochemical assays demonstrated the ability of Co(II) complexes 1-4 to induce significant cytotoxicity in A549 lung cancer cells with IC 50 values of 32.14 ± 1.3 μM, 3.14 ± 0.16 μM, 15.78 ± 0.72 μM and 18.45 ± 0.92 μM, and in MDA-MB-231 breast cancer cells with IC 50 values of 20.42 ± 0.92 μM, 0.41 ± 0.02 μM, 2.31 ± 0.12 μM and 9.67 ± 0.35 μM, respectively. |
