Overexpression of IGF-1 During Early Development Expands the Number of Mammary Stem Cells and Primes them for Transformation.

Autor:	Luo L; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, Bryan, TX, USA.; Biomedical Sciences Graduate Program, Texas A&M University College of Medicine, College Station, TX, USA., Santos A; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, Bryan, TX, USA., Konganti K; Texas A&M Institute for Genome Sciences & Society, Texas A&M University, College Station, TX, USA., Hillhouse A; Texas A&M Institute for Genome Sciences & Society, Texas A&M University, College Station, TX, USA., Lambertz IU; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, Bryan, TX, USA., Zheng Y; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, Bryan, TX, USA., Gunaratna RT; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, Bryan, TX, USA.; Genetics, Texas A&M University Interdisciplinary Degree Programs, College Station, TX, USA., Threadgill DW; Texas A&M Institute for Genome Sciences & Society, Texas A&M University, College Station, TX, USA., Fuchs-Young RS; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, Bryan, TX, USA.
Jazyk:	angličtina
Zdroj:	Stem cells (Dayton, Ohio) [Stem Cells] 2022 Mar 31; Vol. 40 (3), pp. 273-289.
DOI:	10.1093/stmcls/sxab018
Abstrakt:	Insulin-like growth factor I (IGF-1) has been implicated in breast cancer due to its mitogenic and anti-apoptotic effects. Despite substantial research on the role of IGF-1 in tumor progression, the relationship of IGF-1 to tissue stem cells, particularly in mammary tissue, and the resulting tumor susceptibility has not been elucidated. Previous studies with the BK5.IGF-1 transgenic (Tg) mouse model reveals that IGF-1 does not act as a classical, post-carcinogen tumor promoter in the mammary gland. Pre-pubertal Tg mammary glands display increased numbers and enlarged sizes of terminal end buds, a niche for mammary stem cells (MaSCs). Here we show that MaSCs from both wild-type (WT) and Tg mice expressed IGF-1R and that overexpression of Tg IGF-1 increased numbers of MaSCs by undergoing symmetric division, resulting in an expansion of the MaSC and luminal progenitor (LP) compartments in pre-pubertal female mice. This expansion was maintained post-pubertally and validated by mammosphere assays in vitro and transplantation assays in vivo. The addition of recombinant IGF-1 promoted, and IGF-1R downstream inhibitors decreased mammosphere formation. Single-cell transcriptomic profiles generated from 2 related platforms reveal that IGF-1 stimulated quiescent MaSCs to enter the cell cycle and increased their expression of genes involved in proliferation, plasticity, tumorigenesis, invasion, and metastasis. This study identifies a novel, pro-tumorigenic mechanism, where IGF-1 increases the number of transformation-susceptible carcinogen targets during the early stages of mammary tissue development, and "primes" their gene expression profiles for transformation. (© The Author(s) 2022. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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