Fatty Acid Supplementation During in vitro Embryo Production Determines Cryosurvival Characteristics of Bovine Blastocysts.

Autor: Aardema H; Department of Population Health Sciences - Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., Bertijn I; Department of Population Health Sciences - Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., van Tol H; Department of Population Health Sciences - Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., Rijneveld A; Department of Population Health Sciences - Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., Vernooij J; Department of Population Health Sciences - Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., Gadella BM; Department of Population Health Sciences - Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., Vos P; Department of Population Health Sciences - Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.
Jazyk: angličtina
Zdroj: Frontiers in cell and developmental biology [Front Cell Dev Biol] 2022 Mar 09; Vol. 10, pp. 837405. Date of Electronic Publication: 2022 Mar 09 (Print Publication: 2022).
DOI: 10.3389/fcell.2022.837405
Abstrakt: In vitro production (IVP) embryos have a reduced quality and poor cryotolerance in comparison to in vivo embryos. This study investigated whether free fatty acid (FFA) conditions, fatty acid free (FAF)- synthetic oviduct fluid (SOF) without or with 25 μM of saturated stearic (C 18:0 ) or unsaturated oleic (C 18:1 ) acid during the first 5 IVP days, relate to quality and cryosurvival of day 8 blastocysts. Apart from the blastocyst scores, both 1) number and size of lipid droplets of fresh blastocysts and 2) total number and apoptotic and necrotic cells, before and after freezing-thawing, were scored by confocal microscopy. Blastocyst rates were significantly lower in the FAF SOF condition in comparison to other groups. Interestingly, blastocysts originating from the C 18:1 group, with a significantly higher lipid content, and blastocysts from the FAF SOF group demonstrated a high cryosurvival rate (70.1 and 67.4%, respectively) comparable with in vivo blastocysts (68%), in contrast to the poor cryosurvival of C 18:0 exposed embryos (17.6%). In all freeze-thawed embryos the average amount of apoptotic and necrotic cells increased albeit that the C 18:0 condition rates were higher (43.2%) when compared to C 18:1 (26.0%) and FAF SOF conditions (26.5%). The current data show that FFA administered during early embryonic development significantly affect the cryotolerance of blastocysts.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Aardema, Bertijn, van Tol, Rijneveld, Vernooij, Gadella and Vos.)
Databáze: MEDLINE
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