Renoprotective effect of bone marrow mesenchymal stem cells with hyaluronic acid against adriamycin- induced kidney fibrosis via inhibition of Wnt/β-catenin pathway.

Autor: Serag WM; Chemistry Department, Faculty of Science, Suez University, Suez, Egypt., Barakat N; Urology and Nephrology Center, Mansoura University, Mansoura, Egypt., Elshehabi ME; Chemistry Department, Faculty of Science, Suez University, Suez, Egypt., Hafez HS; Zoology Department, Faculty of Science, Suez University, Suez, Egypt., Zahran F; Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2022 May 15; Vol. 207, pp. 741-749. Date of Electronic Publication: 2022 Mar 27.
DOI: 10.1016/j.ijbiomac.2022.03.156
Abstrakt: Aim: The current study aimed to explore the pretreatment of bone marrow mesenchymal stem cells (BMSCs) with hyaluronic acid (HA) on renal fibrosis in Adriamycin- induced CKD in rats.
Material and Methods: Sixty male SD rats were alienated into 4 equal groups; The control group: rats received two saline injections at 1 and 14 days, adriamycin (ADR) group: rats were injected i.v. twice via tail vein at day one and after 2 weeks, BMSCs group; rats were injected i.v. twice after 5 days of each ADR injection, and HA+BMSCs; rats were i.v. injected twice with BMSCs pretreated with 1 mg/ml HA after 5 days of each ADR injection. Protective role of BMSCs on renal function and morphology was detected using biochemical analysis, molecular studies, histopathological, and immunohistohemical investigations.
Results: Pretreatment of BMSCs with HA showed significant decrease in KIM-1, and increase in serum albumin compared to CKD group (p <0.05). Moreover, it reduced the expression of the apoptotic marker Caspase-3, the inflammatory markers TNF and IL-6, and the fibrotic markers Wnt7a, β-catenin, and fibronectin1 than the CKD group (p < 0.05).
Conclusion: The current outcomes suggested that BMSCs preconditioned with HA could attenuate the renal fibrosis in adriamycin- induced CKD.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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