SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2, and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet-plasma membrane tethering.

Autor:	Krawczyk HE; Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Department of Plant Biochemistry, University of Göttingen, Göttingen, Germany., Sun S; Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Department of Plant Biochemistry, University of Göttingen, Göttingen, Germany., Doner NM; Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Yan Q; Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Molecular Biology of Plant-Microbe Interactions Research Group, University of Göttingen, Göttingen, Germany., Lim MSS; Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Department of Plant Biochemistry, University of Göttingen, Göttingen, Germany., Scholz P; Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Department of Plant Biochemistry, University of Göttingen, Göttingen, Germany., Niemeyer PW; Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Department of Plant Biochemistry, University of Göttingen, Göttingen, Germany., Schmitt K; Institute for Microbiology and Genetics and Göttingen Center for Molecular Biosciences (GZMB) and Service Unit LCMS Protein Analytics, Department for Molecular Microbiology and Genetics, University of Göttingen, Göttingen, Germany.; Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany., Valerius O; Institute for Microbiology and Genetics and Göttingen Center for Molecular Biosciences (GZMB) and Service Unit LCMS Protein Analytics, Department for Molecular Microbiology and Genetics, University of Göttingen, Göttingen, Germany.; Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany., Pleskot R; Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic., Hillmer S; Electron Microscopy Core Facility, Heidelberg University, Heidelberg, Germany., Braus GH; Institute for Microbiology and Genetics and Göttingen Center for Molecular Biosciences (GZMB) and Service Unit LCMS Protein Analytics, Department for Molecular Microbiology and Genetics, University of Göttingen, Göttingen, Germany.; Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany., Wiermer M; Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Molecular Biology of Plant-Microbe Interactions Research Group, University of Göttingen, Göttingen, Germany.; Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany., Mullen RT; Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada., Ischebeck T; Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Department of Plant Biochemistry, University of Göttingen, Göttingen, Germany.; Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.; Institute of Plant Biology and Biotechnology (IBBP), Green Biotechnology, University of Münster, Münster, Germany.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2022 May 24; Vol. 34 (6), pp. 2424-2448.
DOI:	10.1093/plcell/koac095
Abstrakt:	Membrane contact sites (MCSs) are interorganellar connections that allow for the direct exchange of molecules, such as lipids or Ca2+ between organelles, but can also serve to tether organelles at specific locations within cells. Here, we identified and characterized three proteins of Arabidopsis thaliana that form a lipid droplet (LD)-plasma membrane (PM) tethering complex in plant cells, namely LD-localized SEED LD PROTEIN (SLDP) 1 and SLDP2 and PM-localized LD-PLASMA MEMBRANE ADAPTOR (LIPA). Using proteomics and different protein-protein interaction assays, we show that both SLDPs associate with LIPA. Disruption of either SLDP1 and SLDP2 expression, or that of LIPA, leads to an aberrant clustering of LDs in Arabidopsis seedlings. Ectopic co-expression of one of the SLDPs with LIPA is sufficient to reconstitute LD-PM tethering in Nicotiana tabacum pollen tubes, a cell type characterized by dynamically moving LDs in the cytosolic streaming. Furthermore, confocal laser scanning microscopy revealed both SLDP2.1 and LIPA to be enriched at LD-PM contact sites in seedlings. These and other results suggest that SLDP and LIPA interact to form a tethering complex that anchors a subset of LDs to the PM during post-germinative seedling growth in Arabidopsis. (© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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