Filopodia rotate and coil by actively generating twist in their actin shaft.

Autor: Leijnse N; Niels Bohr Institute, University of Copenhagen, 2100, Copenhagen, Denmark., Barooji YF; Niels Bohr Institute, University of Copenhagen, 2100, Copenhagen, Denmark., Arastoo MR; Niels Bohr Institute, University of Copenhagen, 2100, Copenhagen, Denmark., Sønder SL; Membrane Integrity, Danish Cancer Society Research Center, Strandboulevarden 49, 2100, Copenhagen, Denmark., Verhagen B; Niels Bohr Institute, University of Copenhagen, 2100, Copenhagen, Denmark., Wullkopf L; Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Ole Maaløes Vej 5, 2200, Copenhagen, Denmark., Erler JT; Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Ole Maaløes Vej 5, 2200, Copenhagen, Denmark., Semsey S; Niels Bohr Institute, University of Copenhagen, 2100, Copenhagen, Denmark., Nylandsted J; Membrane Integrity, Danish Cancer Society Research Center, Strandboulevarden 49, 2100, Copenhagen, Denmark.; Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3C, 2200, Copenhagen, Denmark., Oddershede LB; Niels Bohr Institute, University of Copenhagen, 2100, Copenhagen, Denmark., Doostmohammadi A; Niels Bohr Institute, University of Copenhagen, 2100, Copenhagen, Denmark. doostmohammadi@nbi.ku.dk., Bendix PM; Niels Bohr Institute, University of Copenhagen, 2100, Copenhagen, Denmark. bendix@nbi.ku.dk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Mar 28; Vol. 13 (1), pp. 1636. Date of Electronic Publication: 2022 Mar 28.
DOI: 10.1038/s41467-022-28961-x
Abstrakt: Filopodia are actin-rich structures, present on the surface of eukaryotic cells. These structures play a pivotal role by allowing cells to explore their environment, generate mechanical forces or perform chemical signaling. Their complex dynamics includes buckling, pulling, length and shape changes. We show that filopodia additionally explore their 3D extracellular space by combining growth and shrinking with axial twisting and buckling. Importantly, the actin core inside filopodia performs a twisting or spinning motion which is observed for a range of cell types spanning from earliest development to highly differentiated tissue cells. Non-equilibrium physical modeling of actin and myosin confirm that twist is an emergent phenomenon of active filaments confined in a narrow channel which is supported by measured traction forces and helical buckles that can be ascribed to accumulation of sufficient twist. These results lead us to conclude that activity induced twisting of the actin shaft is a general mechanism underlying fundamental functions of filopodia.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz: