A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines.
| Autor: | Amador-Álvarez A; Department of Cell Biology, Faculty of Biology, Universidad de Sevilla, 41012 Sevilla, Spain.; Instituto de Biomedicina de Sevilla (IBiS), Universidad de Sevilla/CSIC/Junta de Andalucía, 41013 Sevilla, Spain., Gómez-Muñoz MA; Instituto de Biomedicina de Sevilla (IBiS), Universidad de Sevilla/CSIC/Junta de Andalucía, 41013 Sevilla, Spain.; Department of Medical Physiology and Biophysics, Universidad de Sevilla, 41012 Sevilla, Spain., Rodríguez-Prieto I; Instituto de Biomedicina de Sevilla (IBiS), Universidad de Sevilla/CSIC/Junta de Andalucía, 41013 Sevilla, Spain., Pardal R; Instituto de Biomedicina de Sevilla (IBiS), Universidad de Sevilla/CSIC/Junta de Andalucía, 41013 Sevilla, Spain.; Department of Medical Physiology and Biophysics, Universidad de Sevilla, 41012 Sevilla, Spain., Vega FM; Department of Cell Biology, Faculty of Biology, Universidad de Sevilla, 41012 Sevilla, Spain.; Instituto de Biomedicina de Sevilla (IBiS), Universidad de Sevilla/CSIC/Junta de Andalucía, 41013 Sevilla, Spain. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2022 Mar 23; Vol. 3 (2), pp. 101260. Date of Electronic Publication: 2022 Mar 23 (Print Publication: 2022). |
| DOI: | 10.1016/j.xpro.2022.101260 |
| Abstrakt: | The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019). Competing Interests: The authors declare no competing interests (© 2022 The Author(s).) |
| Databáze: | MEDLINE |
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