Comparison of the Antifungal Efficacy of Four Concentrations of Minthostachys mollis (Muña) Essential Oil against Candida albicans: An In Vitro Study.

Autor:	Huamaní K; Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Lima, Peru., Vilchez L; Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Lima, Peru., Mauricio F; Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Lima, Peru., Jáuregui H; Academic Department, Faculty of Medicine, Universidad Nacional Federico Villarreal, Lima, Peru., Munive-Degregori A; Postgraduate Department, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, Peru., Mayta-Tovalino F; Frank Mayta-Tovalino, Vicerrectorado de Investigación, Universidad San Ignacio de Loyola (USIL), Lima, Peru, Phone: +51 1 317 1000, e-mail: fmayta@usil.edu.pe.
Jazyk:	angličtina
Zdroj:	The journal of contemporary dental practice [J Contemp Dent Pract] 2021 Nov 01; Vol. 22 (11), pp. 1227-1231. Date of Electronic Publication: 2021 Nov 01.
Abstrakt:	Aim and Objective: This paper aims to compare the antifungal efficacy of four concentrations of Minthostachys mollis essential oil (MEO) against Candida albicans ATCC 10231. Materials and Methods: This study was conducted in vitro . Ten kilograms of M. mollis (Muña) were collected in the city of Tarma, Peru. The plant was then dried in the shade at room temperature (21°C), and the essential oil was obtained through distillation. C. albicans ATCC 10231 was cultured at a MacFarland scale of 0.5, which corresponds to a concentration of 3 × 10 8 CFU/mL. Each plate was filled with one of the four MEO concentrations (25, 50, 75, or 100%), dimethyl sulfoxide (negative control), or fluconazole (positive control), a known antifungal agent. After incubation, each plate was examined using the Kirby-Bauer method. Results: Compared to MEO 25%, MEO 50%, and MEO 75%, MEO 100% had the highest antifungal efficacy at 24, 48, and 72 hours of evaluation, with an average of 18.9 ± 0.7, 18.2 ± 0.7, and 17.0 ± 0.4 mm, respectively. However, fluconazole had higher efficacy (27.9 ± 0.5, 27.5 ± 0.5, and 23.7 ± 0.7 mm, respectively). Post hoc analysis showed that there were significant differences between all concentrations of the MEO groups and their respective positive and negative control groups ( p <0.001). Conclusion: Among the MEO groups, the pure concentration (MEO 100%) had the highest antifungal efficacy. However, fluconazole presented greater efficacy, and the differences were statistically significant. Clinical Significance: This research allowed to know the efficacy of this natural resource against one of the most prevalent fungi in the oral cavity. Therefore, a line of research could be opened to deepen its potential benefits for oral health.
Databáze:	MEDLINE
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