Biogenic silver nanoparticles reduce Toxoplasma gondii infection and proliferation in RAW 264.7 macrophages by inducing tumor necrosis factor-alpha and reactive oxygen species production in the cells.

Autor: Arruda da Silva Sanfelice R; Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil., Silva TF; Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil., Tomiotto-Pellissier F; Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil; Carlos Chagas Institute (ICC/FIOCRUZ/PR), Curitiba, PR, Brazil., Bortoleti BTDS; Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil; Carlos Chagas Institute (ICC/FIOCRUZ/PR), Curitiba, PR, Brazil., Lazarin-Bidóia D; Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil., Scandorieiro S; Laboratory of Basic and Applied Bacteriology, Department of Microbiology, State University of Londrina, PR, Brazil., Nakazato G; Laboratory of Basic and Applied Bacteriology, Department of Microbiology, State University of Londrina, PR, Brazil., de Barros LD; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445 Km 380, 86057-970, Londrina, PR, Brazil., Garcia JL; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445 Km 380, 86057-970, Londrina, PR, Brazil., Verri WA; Laboratory of Pain, Inflammation, Neuropathy, and Cancer, Department of Pathology, Center of Biological Sciences, Londrina State University, Londrina, PR, Brazil., Conchon-Costa I; Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil., Pavanelli WR; Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil., Costa IN; Laboratory of Immunoparasitology of Neglected Diseases and Cancer - LIDNC, State University of Londrina, PR, Brazil. Electronic address: idessania@hotmail.com.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2022 Jul-Aug; Vol. 24 (5), pp. 104971. Date of Electronic Publication: 2022 Mar 25.
DOI: 10.1016/j.micinf.2022.104971
Abstrakt: Owing to the serious adverse effects caused by pyrimethamine and sulfadiazine, the drugs commonly used to treat toxoplasmosis, there is a need for treatment alternatives for this disease. Nanotechnology has enabled significant advances toward this goal. This study was conducted to evaluate the activity of biogenic silver nanoparticles (AgNp-Bio) in RAW 264.7 murine macrophages infected with the RH strain of Toxoplasma gondii. The macrophages were infected with T. gondii tachyzoites and then treated with various concentrations of AgNp-Bio. The cells were evaluated by microscopy, and culture supernatants were collected for ELISA determination of their cytokine concentration. Treatment with 6 μM AgNp-Bio reduced the infection and parasite load in infected RAW 264.7 macrophages without being toxic to the cells. The treatment also induced the synthesis of reactive oxygen species and tumor necrosis factor-alpha (both pro-inflammatory mediators), which resulted in ultrastructural changes in the tachyzoites and their intramacrophagic destruction. Our findings suggest that AgNp-Bio affect T. gondii tachyzoites by activating microbicidal and pro-inflammatory mechanisms and may be a potential alternative treatment for toxoplasmosis.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest associated with this work.
(Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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