Bone resorption improvement by conditioned medium of stem cells from human exfoliated deciduous teeth in ovariectomized mice.

Autor: Maeda A; Department of Dentistry for Children and Disabled Individuals, Faculty of Dental Medicine and Graduate School of Dental Medicine, Hokkaido University, Sapporo, Hokkaido 060-8586, Japan., Kikuiri T; Department of Dentistry for Children and Disabled Individuals, Faculty of Dental Medicine and Graduate School of Dental Medicine, Hokkaido University, Sapporo, Hokkaido 060-8586, Japan.; Department of Pediatric Dentistry, Nihon University School of Dentistry, Tokyo 101-8310, Japan., Yoshimura Y; Department of Pharmacology, Faculty of Dental Medicine and Graduate School of Dental Medicine, Hokkaido University, Sapporo, Hokkaido 060-8586, Japan., Yawaka Y; Department of Dentistry for Children and Disabled Individuals, Faculty of Dental Medicine and Graduate School of Dental Medicine, Hokkaido University, Sapporo, Hokkaido 060-8586, Japan., Shirakawa T; Department of Pediatric Dentistry, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
Jazyk: angličtina
Zdroj: Experimental and therapeutic medicine [Exp Ther Med] 2022 Apr; Vol. 23 (4), pp. 299. Date of Electronic Publication: 2022 Feb 21.
DOI: 10.3892/etm.2022.11228
Abstrakt: Stem cells from human exfoliated deciduous teeth (SHED) are mesenchymal stem cells with multipotent differentiation potential present in the dental pulp tissue of the deciduous teeth. SHED produce secretions that have immunomodulatory and regenerative functions. In the present study, we investigated the effects of SHED-conditioned medium (SHED-CM) on osteopenia induced by the ovariectomy (OVX) phenotype and its corresponding immunological changes. Eleven-week-old female C3H/HeJ mice were subjected to OVX. SHED-CM was administered intraperitoneally in these mice for 4 weeks starting immediately after OVX. SHED-CM improved bone mass after OVX and elevated the polarization of M2 macrophages in the peritoneal cavity. SHED-CM also suppressed an OVX-induced increase in interferon-γ (INF-γ) and interleukin-17 (IL-17) concentrations in the peripheral blood. Inhibition of M2 macrophage polarization with neutralizing antibodies did not reduce the concentration of IFN-γ and IL-17 in peripheral blood, which were increased by OVX, and did not alleviate osteopenia induced by the OVX phenotype. Mechanistically, these findings suggest that SHED-CM alleviates bone resorption by suppressing the activation of IFN-γ and IL-17 cells by polarizing M2 macrophages. In conclusion, our data indicate that SHED-CM contains active secretions that may have promising efficacy to ameliorate OVX-induced osteopenia. We suggest that SHED-CM has the potential to be used as a novel therapeutic agent to inhibit osteoporosis.
Competing Interests: The authors declare that they have no competing interest.
(Copyright: © Maeda et al.)
Databáze: MEDLINE
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