Immunomodulatory Microneedle Patch for Periodontal Tissue Regeneration.

Autor:	Zhang X; Department of Bioengineering, University of California, Los Angeles, CA 90095, United States., Hasani-Sadrabadi MM; Department of Bioengineering, University of California, Los Angeles, CA 90095, United States., Zarubova J; Department of Bioengineering, University of California, Los Angeles, CA 90095, United States., Dashtimighadam E; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-3290 United States., Haghniaz R; Department of Bioengineering, University of California, Los Angeles, CA 90095, United States.; Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064 USA., Khademhosseini A; Department of Bioengineering, University of California, Los Angeles, CA 90095, United States.; Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064 USA., Butte MJ; Department of Pediatrics, Division of Immunology, Allergy, and Rheumatology, University of California Los Angeles, Los Angeles, CA 90095, United States.; Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095, USA., Moshaverinia A; Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, California 90095, United States., Aghaloo T; Division of Diagnostic and Surgical Sciences, School of Dentistry, University of California, Los Angeles, Los Angeles, CA 90095, United States., Li S; Department of Bioengineering, University of California, Los Angeles, CA 90095, United States.
Jazyk:	angličtina
Zdroj:	Matter [Matter] 2022 Feb 02; Vol. 5 (2), pp. 666-682. Date of Electronic Publication: 2021 Dec 08.
DOI:	10.1016/j.matt.2021.11.017
Abstrakt:	Periodontal diseases are caused by microbial infection and the recruitment of destructive immune cells. Current therapies mainly deal with bacteria elimination, but the regeneration of periodontal tissues remains a challenge. Here we developed a modular microneedle (MN) patch that delivered both antibiotic and cytokines into the local gingival tissue to achieve immunomodulation and tissue regeneration. This MN patch included a quickly dissolvable gelatin membrane for an immediate release of tetracycline and biodegradable GelMA MNs that contained tetracycline-loaded poly(lactic-co-glycolic acid) nanoparticles and cytokine-loaded silica microparticles for a sustained release. Antibiotic release completely inhibited bacteria growth, and the release of IL-4 and TGF-β induced the repolarization of anti-inflammatory macrophages and the formation of regulatory T cells in vitro . In vivo delivery of MN patch into periodontal tissues suppressed proinflammatory factors and promoted pro-regenerative signals and tissue healing, which demonstrated the therapeutic potential of local immunomodulation for tissue regeneration. Competing Interests: Declaration of Interests: S.L., M.M.H.-S., T.A., and A.M. have patent applications (periodontal micropatch and uses thereof, U.S. Provisional Patent Application PCT/US20/58069) related to the current study and, thus, may have related financial interests. The other authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.
Databáze:	MEDLINE
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