Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62.

Autor: Yu G; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.; Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.; KAUST Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia., Matny O; Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA., Champouret N; The Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UK, UK.; Syngenta Flowers, Downers Grove, IL, 60515, USA., Steuernagel B; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Moscou MJ; The Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UK, UK., Hernández-Pinzón I; The Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UK, UK., Green P; The Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UK, UK., Hayta S; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Smedley M; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Harwood W; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Kangara N; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Yue Y; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Gardener C; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Banfield MJ; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK., Olivera PD; Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA., Welchin C; Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA., Simmons J; Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA., Millet E; Institute for Cereal Crops Improvement, Tel Aviv University, Tel Aviv, Israel., Minz-Dub A; Institute for Cereal Crops Improvement, Tel Aviv University, Tel Aviv, Israel., Ronen M; Institute for Cereal Crops Improvement, Tel Aviv University, Tel Aviv, Israel., Avni R; Institute for Cereal Crops Improvement, Tel Aviv University, Tel Aviv, Israel.; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel.; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)., Seeland, Germany., Sharon A; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Patpour M; Department of Agroecology, Aarhus University, Forsøgsvej 1, DK-4200, Slagelse, Denmark., Justesen AF; Department of Agroecology, Aarhus University, Forsøgsvej 1, DK-4200, Slagelse, Denmark., Jayakodi M; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany., Himmelbach A; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany., Stein N; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany.; Center of integrated Breeding Research (CiBreed), Department of Crop Sciences, Georg-August-University, Von Siebold Str. 8, 37075, Göttingen, Germany., Wu S; Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA., Poland J; Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA., Ens J; Crop Development Centre, Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada., Pozniak C; Crop Development Centre, Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada., Karafiátová M; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, 779 00, Olomouc, Czech Republic., Molnár I; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, 779 00, Olomouc, Czech Republic.; Agricultural Institute, Centre for Agricultural Research, ELKH, Martonvásár, 2462, Hungary., Doležel J; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, 779 00, Olomouc, Czech Republic., Ward ER; The Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UK, UK.; 2Blades Foundation, Evanston, IL, USA.; AgBiome, Inc., Research Triangle Park, NC, 27709, USA., Reuber TL; 2Blades Foundation, Evanston, IL, USA.; Alliance Management at Enko Chem, 62 Maritime Dr, Mystic, CT, 06355, USA., Jones JDG; The Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UK, UK., Mascher M; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany.; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103, Leipzig, Germany., Steffenson BJ; Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA. bsteffen@umn.edu., Wulff BBH; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. brande.wulff@kaust.edu.sa.; Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia. brande.wulff@kaust.edu.sa.; KAUST Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia. brande.wulff@kaust.edu.sa.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Mar 25; Vol. 13 (1), pp. 1607. Date of Electronic Publication: 2022 Mar 25.
DOI: 10.1038/s41467-022-29132-8
Abstrakt: The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.
(© 2022. The Author(s).)
Databáze: MEDLINE
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