The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation.

Autor:	Beukema M; Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands. Electronic address: m.beukema@umcg.nl., Jermendi É; Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands. Electronic address: eva.jermendi@wur.nl., Oerlemans MMP; Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands. Electronic address: m.m.p.oerlemans@umcg.nl., Logtenberg MJ; Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands. Electronic address: madelon.logtenberg@wur.nl., Akkerman R; Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands. Electronic address: r.akkerman@umcg.nl., An R; Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, the Netherlands. Electronic address: ran.an@wur.nl., van den Berg MA; DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, the Netherlands. Electronic address: marco.van.den.berg@DSM.com., Zoetendal EG; Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, the Netherlands. Electronic address: erwin.zoetendal@wur.nl., Koster T; Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands. Electronic address: t.koster@umcg.nl., Kong C; Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands. Electronic address: c.kong@umcg.nl., Faas MM; Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands. Electronic address: m.m.faas@umcg.nl., Schols HA; Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands. Electronic address: henk.schols@wur.nl., de Vos P; Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands. Electronic address: p.de.vos@umcg.nl.
Jazyk:	angličtina
Zdroj:	Carbohydrate polymers [Carbohydr Polym] 2022 Jun 15; Vol. 286, pp. 119280. Date of Electronic Publication: 2022 Feb 23.
DOI:	10.1016/j.carbpol.2022.119280
Abstrakt:	Pectins are dietary fibres that modulate T cell immunity, microbiota composition, and fermentation profiles, but how this is influenced by the degree of methyl-esterification (DM) and degree-of-blockiness (DB) of pectin is unknown. Here, we demonstrate that supplementation of DM19(high-DB), DM49(low-DB) and DM43(high-DB) pectins at a low dose increased the frequencies of intestinal T-helper (Th)1 and Th2 cells after 1 week of pectin supplementation in mice, whereas DM18(low-DB) did not. After 4 weeks of supplementation with those pectins, Th1 and Th2 frequencies returned to control levels, whereas Rorγt + regulatory T-cell frequencies increased. These structure-dependent effects could derive from induced shifts in microbiota composition that differed between DM18(low-DB) pectin and the other pectins. T-cell-modulating effects were not short-chain-fatty acid-dependent, but rather through an increase in Aryl-hydrocarbon-receptor-activating components. Thus, pectins with a specific combination of DM and DB have an impact on intestinal T cell-immunity in mice, when supplemented at a low dose. (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Full Text from ScienceDirect