| Autor: |
Lee MD; Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA., Creagh JW; Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA., Fredericks LR; Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA., Crabtree AM; Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA., Patel JS; Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA.; Center for Modeling Complex Interactions, University of Idaho, Moscow, ID 83844, USA., Rowley PA; Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA. |
| Abstrakt: |
Mycoviruses are widely distributed across fungi, including the yeasts of the Saccharomycotina subphylum. This manuscript reports the first double-stranded RNA (dsRNA) virus isolated from Pichia membranifaciens. This novel virus has been named Pichia membranifaciens virus L-A (PmV-L-A) and is a member of the Totiviridae . PmV-L-A is 4579 bp in length, with RNA secondary structures similar to the packaging, replication, and frameshift signals of totiviruses that infect Saccharomycotina yeasts. PmV-L-A was found to be part of a monophyletic group within the I-A totiviruses, implying a shared ancestry between mycoviruses isolated from the Pichiaceae and Saccharomycetaceae yeasts. Energy-minimized AlphaFold2 molecular models of the PmV-L-A Gag protein revealed structural conservation with the Gag protein of Saccharomyces cerevisiae virus L-A (ScV-L-A). The predicted tertiary structure of the PmV-L-A Pol and other homologs provided a possible mechanism for totivirus RNA replication due to structural similarities with the RNA-dependent RNA polymerases of mammalian dsRNA viruses. Insights into the structure, function, and evolution of totiviruses gained from yeasts are essential because of their emerging role in animal disease and their parallels with mammalian viruses. |
