Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes.

Autor: Antoshkina IV; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, Russia., Glazkova DV; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, Russia., Urusov FA; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, Russia.; Izmerov Research Institute of Occupational Health, 105275 Moscow, Russia., Bogoslovskaya EV; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, Russia., Shipulin GA; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, Russia.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2022 Mar 04; Vol. 14 (3). Date of Electronic Publication: 2022 Mar 04.
DOI: 10.3390/v14030528
Abstrakt: Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly improved and facilitated by different selection techniques. Here, we describe a comparison between techniques based on K1L, F13L and D4R genes.
Databáze: MEDLINE
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