Bioactive Compounds and Antifungal Activity of Leaves and Fruits Methanolic Extracts of Ziziphus spina-christi L.

Autor: El-Shahir AA; Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena 83523, Egypt., El-Wakil DA; Biology Department, Facutly of Science, Jazan University, Jazan 82817, Saudi Arabia.; Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt., Abdel Latef AAH; Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena 83523, Egypt., Youssef NH; Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena 83523, Egypt.
Jazyk: angličtina
Zdroj: Plants (Basel, Switzerland) [Plants (Basel)] 2022 Mar 11; Vol. 11 (6). Date of Electronic Publication: 2022 Mar 11.
DOI: 10.3390/plants11060746
Abstrakt: Zizyphus spina-christi L. has antimicrobial properties because of the presence of biologically active compounds. Alternaria is an opportunistic pathogen that causes leaf spots, rots, and blights on a variety of plant parts. This study aimed to reduce the usage of synthetically derived fungicides. Identification of the bioactive components present in leaves and fruits methanolic extracts of Z. spina-christi was performed using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The efficacy of the two methanol extracts was tested against (a) in vitro fungal growth and (b) pathogenicity control on non-wounded and wounded tomato fruits. The results revealed that gallic acid and ellagic acid were the major components in leaves extract while quercetin was the major component in fruits extract. In addition, Phenol, 2,5-bis(1,1-dimethylethyl) (40.24%) and Decane, 2-methyl-(18.53%) were the most abundant components in the leaf extract, and the presence of D-mannonic acid, 2,3,5,6-tetrakis-o-(trimethylsilyl), and γ-lactone (22.72%) were major components in fruits extract. The methanolic extracts of Z. spina-christi leaves and fruits demonstrated significant antifungal activity against the growth of Alternaria alternata , A. citri , and A. radicina with variable inhibition percentages at different concentrations. Pathogenicity was increased when the skin was injured, as expected. Both extracts reduced the percentage of infected fruits.
Databáze: MEDLINE