First Detection of Francisella halioticida Infecting a Wild Population of Blue Mussels Mytilus edulis in the United Kingdom.

Autor:	Cano I; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK., Parker A; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK., Ward GM; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK.; Department of Life Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD, UK., Green M; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK., Ross S; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK., Bignell J; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK., Daumich C; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK., Kerr R; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK., Feist SW; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK., Batista FM; Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK.
Jazyk:	angličtina
Zdroj:	Pathogens (Basel, Switzerland) [Pathogens] 2022 Mar 08; Vol. 11 (3). Date of Electronic Publication: 2022 Mar 08.
DOI:	10.3390/pathogens11030329
Abstrakt:	In the last decade, declines in the population of wild blue mussels Mytilus edulis in the Tamar estuary (United Kingdom) have been noted. In archived samples collected from 2013 to 2019, between 7% (in 2013) and 18% (in 2019) showed large granulocytoma and haemocytic infiltration in the interstitial tissue of the digestive gland. Four samples were selected for 16S rRNA gene Nanopore sequencing. A consensus sequence of 1449 bp showed nucleotide similarities between 99.93-100% with published sequences of Francisella halioticida . In situ hybridisation (ISH) confirmed the presence of F. halioticida DNA within individual granulocytes of granulocytomas and also in prokaryotic-like inclusion bodies within the digestive epithelial cells. The design of diagnostic tests for surveillance of F. halioticida, including more specific ISH probes and sequencing the genome of the isolates infecting mussels, will shed more light on the pathogenicity and spread of this pathogen.
Databáze:	MEDLINE
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