Comparisons of plasma and fecal pharmacokinetics of danofloxacin and enrofloxacin in healthy and Mannheimia haemolytica infected calves.

Autor: Beyi AF; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.; National Institute of Antimicrobial Resistance Research and Education, Iowa State University, Ames, IA, 50010, USA., Mochel JP; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.; National Institute of Antimicrobial Resistance Research and Education, Iowa State University, Ames, IA, 50010, USA., Magnin G; Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66502, USA., Hawbecker T; College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA., Slagel C; College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA., Dewell G; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA., Dewell R; Center for Food Security/Public Health, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA., Sahin O; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.; National Institute of Antimicrobial Resistance Research and Education, Iowa State University, Ames, IA, 50010, USA., Coetzee JF; Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66502, USA.; Nanotechnology Innovation Center of Kansas State (NICKS) and Institute of Computational Comparative Medicine, Kansas State University, Manhattan, KS, 66502, USA., Zhang Q; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.; National Institute of Antimicrobial Resistance Research and Education, Iowa State University, Ames, IA, 50010, USA., Plummer PJ; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA. pplummer@iastate.edu.; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA. pplummer@iastate.edu.; National Institute of Antimicrobial Resistance Research and Education, Iowa State University, Ames, IA, 50010, USA. pplummer@iastate.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Mar 24; Vol. 12 (1), pp. 5107. Date of Electronic Publication: 2022 Mar 24.
DOI: 10.1038/s41598-022-08945-z
Abstrakt: Danofloxacin and enrofloxacin are fluoroquinolones (FQs) used to treat and control bovine respiratory disease (BRD) complex. While low toxicity, high bactericidal activity, and availability in single and multiple dosing regimens make them preferable, the increasing incidence of FQ-resistance in foodborne pathogens and effects on gut microbiota necessitate evaluating their pharmacokinetics (PKs). The objective of this study was to determine the exposure level of gut microbiota to subcutaneously administered FQs and compare their PKs between plasma and feces in healthy and Mannheimia haemolytica infected calves. A single dose of danofloxacin (8 mg/kg), low dose (7.5 mg/kg), or high dose (12.5 mg/kg) of enrofloxacin was administered to calves. Blood and feces were collected from calves under experimental conditions over 48 h, and FQ concentrations were measured using Ultra High-Pressure Liquid Chromatography. While moderate BRD signs were exhibited in most calves in the infected cohorts, the plasma PKs were similar between healthy and sick calves. However, the fecal danofloxacin concentration was lower in the BRD group (area under concentration-time curve [AUC inf ], BRD median = 2627, healthy median = 2941 h*μg/mL, adj.P = 0.005). The dose normalized plasma and fecal danofloxacin concentrations were higher than those of enrofloxacin and its metabolite ciprofloxacin. Further, FQs had several fold higher overall concentrations in feces than in plasma in both groups. In conclusion, parenterally administered FQs expose gut microbiota to high concentrations of the antibiotics.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje