| Autor: |
Bojanović M; Medical Faculty, University of Niš, 18000 Niš, Serbia.; Clinic of Otorhinolaryngology, University Clinical Center Niš, 18000 Niš, Serbia., Ignjatović A; Medical Faculty, University of Niš, 18000 Niš, Serbia.; Public Health Institute Niš, 18000 Niš, Serbia., Stalević M; Medical Faculty, University of Niš, 18000 Niš, Serbia., Arsić-Arsenijević V; Medical Faculty, University of Belgrade, 11000 Belgrade, Serbia., Ranđelović M; Medical Faculty, University of Niš, 18000 Niš, Serbia.; Public Health Institute Niš, 18000 Niš, Serbia., Gerginić V; Medical Faculty, University of Belgrade, 11000 Belgrade, Serbia., Stojanović-Radić Z; Department of Biology, Faculty of Science and Mathematics, University of Niš, 18000 Niš, Serbia., Stojković O; Department of Biology, Faculty of Science and Mathematics, University of Niš, 18000 Niš, Serbia., Živković-Marinkov E; Medical Faculty, University of Niš, 18000 Niš, Serbia.; Clinic of Otorhinolaryngology, University Clinical Center Niš, 18000 Niš, Serbia., Otašević S; Medical Faculty, University of Niš, 18000 Niš, Serbia.; Public Health Institute Niš, 18000 Niš, Serbia. |
| Abstrakt: |
Species of Aspergillus ( A. ) niger complex and A. flavus complex are predominant molds that are causative agents of otomycoses. The goal of this study was to investigate the clinical presentation, diagnostic procedure, and appearance of relapse in patients with Aspergillus -otomycosis, as well as to determine the biofilm production ability of species isolated in relapse. Thirty patients with laboratory evidenced Aspergillus -otomycosis followed by two check-ups (30 and 60 days after initiation of treatment with antimycotics for local application) were included in the study. For isolation and identification of Aspergillus spp. the standard mycological procedure was applied. Results showed very high sensitivity of microscopy, but 16.7% Aspergillus species required the optimal temperature of 27-28 °C for cultivation. Applied statistical cluster analysis showed a defined specific cluster/group of patients with A. niger complex-otomycosis. Sixty days after diagnosis and treatment initiation, six patients had a relapse, with the same species of Aspergillus genus being the cause. To establish the ability of biofilm production, the modified method described by Pierce and Kvasničková was performed, and all six species isolated in the relapse episode had the ability to produce biofilm. Official criteria and recommendations are needed due to the possibility of misdiagnosis, which leads to the prolongation and complication of the disease. |
