The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance.

Autor: Gu T; Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark., Just J; Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.; Sino-Danish College (SDC), University of Chinese Academy of Sciences, Beijing 100190, China.; Department of Molecular Medicine, Aarhus University Hospital, 8200 Aarhus, Denmark., Stenz KT; Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.; Sino-Danish College (SDC), University of Chinese Academy of Sciences, Beijing 100190, China., Yan Y; Interdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, Denmark., Sieljacks P; Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark., Wang J; Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark., Groennebaek TS; Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark., Jakobsgaard JE; Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark., Rindom E; Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark., Herskind J; Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark., Gravholt A; Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark., Lassen TR; Department of Cardiology, Aarhus University Hospital, 8200 Aarhus, Denmark., Jørgensen M; Department of Engineering, Aarhus University, 8000 Aarhus, Denmark., Bæk R; Department of Clinical Immunology, Aalborg University Hospital, 9100 Aalborg, Denmark., Gutiérrez-Jiménez E; Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark., Iversen NK; Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark., Rasmussen PM; Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark., Nyengaard JR; Sino-Danish College (SDC), University of Chinese Academy of Sciences, Beijing 100190, China.; Core Center for Molecular Morphology, Section for Stereology and Microscopy, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.; Department of Pathology, Aarhus University Hospital, 8200 Aarhus, Denmark., Jørgensen MM; Department of Clinical Immunology, Aalborg University Hospital, 9100 Aalborg, Denmark.; Department of Clinical Medicine, Aalborg University, 9220 Aalborg, Denmark., de Paoli F; Department of Biomedicine-Physiology, Aarhus University, 8000 Aarhus, Denmark., Bøtker HE; Department of Cardiology, Aarhus University Hospital, 8200 Aarhus, Denmark., Kjems J; Interdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, Denmark.; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark., Vissing K; Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark., Drasbek KR; Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.; Core Center for Molecular Morphology, Section for Stereology and Microscopy, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Mar 19; Vol. 23 (6). Date of Electronic Publication: 2022 Mar 19.
DOI: 10.3390/ijms23063334
Abstrakt: Ischemic conditioning and exercise have been suggested for protecting against brain ischemia-reperfusion injury. However, the endogenous protective mechanisms stimulated by these interventions remain unclear. Here, in a comprehensive translational study, we investigated the protective role of extracellular vesicles (EVs) released after remote ischemic conditioning (RIC), blood flow restricted resistance exercise (BFRRE), or high-load resistance exercise (HLRE). Blood samples were collected from human participants before and at serial time points after intervention. RIC and BFRRE plasma EVs released early after stimulation improved viability of endothelial cells subjected to oxygen-glucose deprivation. Furthermore, post-RIC EVs accumulated in the ischemic area of a stroke mouse model, and a mean decrease in infarct volume was observed for post-RIC EVs, although not reaching statistical significance. Thus, circulating EVs induced by RIC and BFRRE can mediate protection, but the in vivo and translational effects of conditioned EVs require further experimental verification.
Databáze: MEDLINE
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