| Autor: |
Mucenski CM, Guerry P, Buesing M, Szarfman A, Trosper J, Walliker D, Watt G, Sangalang R, Ranoa CP, Tuazon M, et. al. |
| Jazyk: |
angličtina |
| Zdroj: |
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 1986 Sep; Vol. 35 (5), pp. 912-20. |
| DOI: |
10.4269/ajtmh.1986.35.912 |
| Abstrakt: |
A radiolabeled synthetic oligonucleotide was evaluated as a diagnostic probe specific for Plasmodium falciparum using blood samples lysed directly on nitrocellulose filters. The probe technique successfully diagnosed malaria in experimentally infected chimpanzees that had 0.001% parasitemias (50 parasites/microliter) as determined by blood smears, and in 1 chimpanzee whose blood smear was negative, but whose blood was culture-positive for P. falciparum. In a double blind study of 50 patient samples from the Philippines, the probe results correlated well with blood smear results when the autoradiographs were read after 4-8 hr exposure. The results indicate that the oligonucleotide probe may be useful in the rapid and specific diagnosis of P. falciparum infection. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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