| Autor: |
Li A; Toulouse Biotechnology Institute (TBI), Centre National de la Recherche Scientifique (CNRS) Toulouse Biotechnology Institute (TBI), French National Research Institute for Agriculture, Food and Environment (INRAE), Institut National des Sciences Appliquées (INSA), Université de Toulouse, 31000 Toulouse, France., Benkoulouche M; Toulouse Biotechnology Institute (TBI), Centre National de la Recherche Scientifique (CNRS) Toulouse Biotechnology Institute (TBI), French National Research Institute for Agriculture, Food and Environment (INRAE), Institut National des Sciences Appliquées (INSA), Université de Toulouse, 31000 Toulouse, France., Ladeveze S; Toulouse Biotechnology Institute (TBI), Centre National de la Recherche Scientifique (CNRS) Toulouse Biotechnology Institute (TBI), French National Research Institute for Agriculture, Food and Environment (INRAE), Institut National des Sciences Appliquées (INSA), Université de Toulouse, 31000 Toulouse, France., Durand J; Toulouse Biotechnology Institute (TBI), Centre National de la Recherche Scientifique (CNRS) Toulouse Biotechnology Institute (TBI), French National Research Institute for Agriculture, Food and Environment (INRAE), Institut National des Sciences Appliquées (INSA), Université de Toulouse, 31000 Toulouse, France., Cioci G; Toulouse Biotechnology Institute (TBI), Centre National de la Recherche Scientifique (CNRS) Toulouse Biotechnology Institute (TBI), French National Research Institute for Agriculture, Food and Environment (INRAE), Institut National des Sciences Appliquées (INSA), Université de Toulouse, 31000 Toulouse, France., Laville E; Toulouse Biotechnology Institute (TBI), Centre National de la Recherche Scientifique (CNRS) Toulouse Biotechnology Institute (TBI), French National Research Institute for Agriculture, Food and Environment (INRAE), Institut National des Sciences Appliquées (INSA), Université de Toulouse, 31000 Toulouse, France., Potocki-Veronese G; Toulouse Biotechnology Institute (TBI), Centre National de la Recherche Scientifique (CNRS) Toulouse Biotechnology Institute (TBI), French National Research Institute for Agriculture, Food and Environment (INRAE), Institut National des Sciences Appliquées (INSA), Université de Toulouse, 31000 Toulouse, France. |
| Abstrakt: |
Among carbohydrate active enzymes, glycoside phosphorylases (GPs) are valuable catalysts for white biotechnologies, due to their exquisite capacity to efficiently re-modulate oligo- and poly-saccharides, without the need for costly activated sugars as substrates. The reversibility of the phosphorolysis reaction, indeed, makes them attractive tools for glycodiversification. However, discovery of new GP functions is hindered by the difficulty in identifying them in sequence databases, and, rather, relies on extensive and tedious biochemical characterization studies. Nevertheless, recent advances in automated tools have led to major improvements in GP mining, activity predictions, and functional screening. Implementation of GPs into innovative in vitro and in cellulo bioproduction strategies has also made substantial advances. Herein, we propose to discuss the latest developments in the strategies employed to efficiently discover GPs and make the best use of their exceptional catalytic properties for glycoside bioproduction. |
