| Autor: |
Jamehdor S; Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 989155432609, Iran., Pajouhanfar S; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA., Saba S; Center for Molecular Medicine & Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USA., Uzan G; INSERM UMR-S-MD 1197, Hôpital Paul Brousse, 94800 Villejuif, France.; Paris-Saclay University, 94800 Villejuif, France., Teimoori A; Department of Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan 6517838738, Iran., Naserian S; INSERM UMR-S-MD 1197, Hôpital Paul Brousse, 94800 Villejuif, France.; Paris-Saclay University, 94800 Villejuif, France.; CellMedEx, 94100 Saint Maur Des Fossés, France. |
| Abstrakt: |
Viruses are one of the most important concerns for human health, and overcoming viral infections is a worldwide challenge. However, researchers have been trying to manipulate viral genomes to overcome various disorders, including cancer, for vaccine development purposes. CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms. This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus. Furthermore, this method can be used to make an effective and precise diagnosis of viral infections. Nevertheless, a valid and scientifically designed CRISPR system is critical to make more effective and accurate changes in viruses. In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation. Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions. |
