Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters.

Autor: Festa M; Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy., Minicozzi V; INFN, Department of Physics, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy., Boccaccio A; Institute of Biophysics, National Research Council, Via De Marini 6, 16149 Genoa, Italy., Lagostena L; Institute of Biophysics, National Research Council, Via De Marini 6, 16149 Genoa, Italy., Gradogna A; Institute of Biophysics, National Research Council, Via De Marini 6, 16149 Genoa, Italy., Qi T; Department of Earth, Environment and Life Sciences (DISTAV), University of Genoa, Viale Benedetto XV 5, 16132 Genoa, Italy., Costa A; Department of Biosciences, University of Milan, 20133 Milan, Italy., Larisch N; Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91058 Erlangen, Germany., Hamamoto S; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan., Pedrazzini E; Institute of Agricultural Biology and Biotechnology, National Research Council, Via Bassini 15, 20133 Milan, Italy., Milenkovic S; Department of Physics, University of Cagliari, 09042 Monserrato, Italy.; IOM-CNR Unità di Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy., Scholz-Starke J; Institute of Biophysics, National Research Council, Via De Marini 6, 16149 Genoa, Italy., Ceccarelli M; Department of Physics, University of Cagliari, 09042 Monserrato, Italy.; IOM-CNR Unità di Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy., Vitale A; Institute of Agricultural Biology and Biotechnology, National Research Council, Via Bassini 15, 20133 Milan, Italy., Dietrich P; Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91058 Erlangen, Germany., Uozumi N; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan., Gambale F; Institute of Biophysics, National Research Council, Via De Marini 6, 16149 Genoa, Italy., Carpaneto A; Institute of Biophysics, National Research Council, Via De Marini 6, 16149 Genoa, Italy.; Department of Earth, Environment and Life Sciences (DISTAV), University of Genoa, Viale Benedetto XV 5, 16132 Genoa, Italy.
Jazyk: angličtina
Zdroj: Cells [Cells] 2022 Mar 08; Vol. 11 (6). Date of Electronic Publication: 2022 Mar 08.
DOI: 10.3390/cells11060921
Abstrakt: A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.
Databáze: MEDLINE
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