| Autor: |
Dulić T; Department of Biochemistry, Faculty of Sciences and Engineering, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland., Svirčev Z; Department of Biochemistry, Faculty of Sciences and Engineering, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland.; University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia., Palanački Malešević T; University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia., Faassen EJ; Wageningen Food Safety Research, Wageningen University and Research, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands.; Aquatic Ecology and Water Quality Management, Wageningen University and Research, Droevendaalsesteeg 3a, 6708 PB Wageningen, The Netherlands., Savela H; Department of Life Technologies, Faculty of Technology, University of Turku, Kiinamyllynkatu 10, 20014 Turku, Finland., Hao Q; Laboratory of Cenozoic Geology and Environment, Institute of Geology and Geophysics, Chinese Academy of Sciences, No. 19, Beitucheng Western Road, Beijing 100029, China., Meriluoto J; Department of Biochemistry, Faculty of Sciences and Engineering, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland.; University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia. |
| Abstrakt: |
Cyanotoxins are a diverse group of bioactive compounds produced by cyanobacteria that have adverse effects on human and animal health. While the phenomenon of cyanotoxin production in aquatic environments is well studied, research on cyanotoxins in terrestrial environments, where cyanobacteria abundantly occur in biocrusts, is still in its infancy. Here, we investigated the potential cyanotoxin production in cyanobacteria-dominated biological loess crusts (BLCs) from three different regions (China, Iran, and Serbia) and in cyanobacterial cultures isolated from the BLCs. The presence of cyanotoxins microcystins, cylindrospermopsin, saxitoxins, and β-N-methylamino-L-alanine was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, while the presence of cyanotoxin-encoding genes ( mcyE , cyrJ , sxtA , sxtG , sxtS , and anaC ) was investigated by polymerase chain reaction (PCR) method. We could not detect any of the targeted cyanotoxins in the biocrusts or the cyanobacterial cultures, nor could we amplify any cyanotoxin-encoding genes in the cyanobacterial strains. The results are discussed in terms of the biological role of cyanotoxins, the application of cyanobacteria in land restoration programs, and the use of cyanotoxins as biosignatures of cyanobacterial populations in loess research. The article highlights the need to extend the field of research on cyanobacteria and cyanotoxin production to terrestrial environments. |
