Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy.

Autor:	Kilroy EA; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United States., Ignacz AC; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United States., Brann KL; School of Biology and Ecology, University of Maine, Orono, United States., Schaffer CE; School of Biology and Ecology, University of Maine, Orono, United States., Varney D; School of Biology and Ecology, University of Maine, Orono, United States., Alrowaished SS; School of Biology and Ecology, University of Maine, Orono, United States., Silknitter KJ; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United States., Miner JN; Department of Chemical and Biomedical Engineering, University of Maine, Orono, United States., Almaghasilah A; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United States., Spellen TL; School of Biology and Ecology, University of Maine, Orono, United States., Lewis AD; School of Biology and Ecology, University of Maine, Orono, United States., Tilbury K; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United States.; Department of Chemical and Biomedical Engineering, University of Maine, Orono, United States., King BL; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United States.; Department of Molecular and Biomedical Sciences, University of Maine, Orono, United States., Kelley JB; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United States.; Department of Molecular and Biomedical Sciences, University of Maine, Orono, United States., Henry CA; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United States.; School of Biology and Ecology, University of Maine, Orono, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2022 Mar 24; Vol. 11. Date of Electronic Publication: 2022 Mar 24.
DOI:	10.7554/eLife.62760
Abstrakt:	Neuromuscular electrical stimulation (NMES) allows activation of muscle fibers in the absence of voluntary force generation. NMES could have the potential to promote muscle homeostasis in the context of muscle disease, but the impacts of NMES on diseased muscle are not well understood. We used the zebrafish Duchenne muscular dystrophy ( dmd ) mutant and a longitudinal design to elucidate the consequences of NMES on muscle health. We designed four neuromuscular stimulation paradigms loosely based on weightlifting regimens. Each paradigm differentially affected neuromuscular structure, function, and survival. Only endurance neuromuscular stimulation (eNMES) improved all outcome measures. We found that eNMES improves muscle and neuromuscular junction morphology, swimming, and survival. Heme oxygenase and integrin alpha7 are required for eNMES-mediated improvement. Our data indicate that neuromuscular stimulation can be beneficial, suggesting that the right type of activity may benefit patients with muscle disease. Competing Interests: EK, AI, KB, CS, DV, SA, KS, JM, AA, TS, AL, KT, BK, JK, CH No competing interests declared (© 2022, Kilroy et al.)
Databáze:	MEDLINE
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