| Autor: |
Ravindranath MH; Department of Hematology and Oncology, Children's Hospital, Los Angeles, CA 90027, USA.; Emeritus Research Scientist at Terasaki Foundation Laboratory, Santa Monica, CA 90064, USA., Ravindranath NM; Norris Dental Science Center, Herman Vostro School of Dentistry, University of Southern California, Los Angeles, CA 90089, USA., El Hilali F; Faculty of Medicine and Pharmacy of Laayoune, Ibn Zohr University, Agadir 70000, Morocco., Selvan SR; Tetracore Inc., Rockville, MD 20850, USA., Filippone EJ; Division of Nephrology, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19145, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Antibodies (Basel, Switzerland) [Antibodies (Basel)] 2022 Mar 02; Vol. 11 (1). Date of Electronic Publication: 2022 Mar 02. |
| DOI: |
10.3390/antib11010018 |
| Abstrakt: |
Serum anti-HLA-I IgG are present in non-alloimmunized males, cancer patients, and transplant recipients. Anti-HLA-I antibodies are also present in intravenous immunoglobulin (IVIg), prepared from the plasma of thousands of healthy donors. However, the HLA-Ia reactivity of IVIg diminishes markedly after passing through HLA-E HC-affinity columns, suggesting that the HLA-I reactivity is due to antibodies formed against HLA-E. Hence, we examined whether anti-HLA-E antibodies can react to HLA-I alleles. Monoclonal IgG antibodies (mAbs) against HCs of two HLA-E alleles were generated in Balb/C mice. The antibodies were analyzed using multiplex bead assays on a Luminex platform for HLA-I reactivity. Beads coated with an array of HLA heterodimers admixed with HCs (LABScreen) were used to examine the binding of IgG to different HLA-Ia (31-HLA-A, 50-HLA-B, and 16-HLA-C) and Ib (2-HLA-E, one each of HLA-F and HLA-G) alleles. A striking diversity in the HLA-Ia and/or HLA-Ib reactivity of mAbs was observed. The number of the mAbs reactive to (1) only HLA-E ( n = 25); (2) all HLA-Ib isomers ( n = 8); (3) HLA-E and HLA-B ( n = 5); (4) HLA-E, HLA-B, and HLA-C ( n = 30); (5) HLA-E, HLA-A*1101, HLA-B, and HLA-C ( n = 83); (6) HLA-E, HLA-A, HLA-B, and HLA-C ( n = 54); and (7) HLA-Ib and HLA-Ia ( n = 8), in addition to four other minor groups. Monospecificity and polyreactivity were corroborated by HLA-E monospecific and HLA-I shared sequences. The diverse HLA-I reactivity of the mAbs are compared with the pattern of HLA-I reactivity of serum-IgG in non-alloimmunized males, cancer patients, and ESKD patients. The findings unravel the diagnostic potential of the HLA-E monospecific-mAbs and immunomodulatory potentials of IVIg highly mimicking HLA-I polyreactive-mAbs. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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