Allelic imbalance of HLA-B expression in human lung cells infected with coronavirus and other respiratory viruses.

Autor: Zhang Y; Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou, China., Sun Y; Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Province Center for Disease Prevention and Control, Hangzhou, China., Zhu H; Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Province Center for Disease Prevention and Control, Hangzhou, China., Hong H; Key Laboratory of Tropical Disease Control of Sun Yat-Sen University, Ministry of Education, Sun Yat-Sen University, Guangzhou, China., Jiang J; Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Province Center for Disease Prevention and Control, Hangzhou, China., Yao P; Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Province Center for Disease Prevention and Control, Hangzhou, China. ppyao@cdc.zj.cn., Liao H; Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou, China. larryhliao@163.com., Zhang Y; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA. yanfengzhang1984@outlook.com.
Jazyk: angličtina
Zdroj: European journal of human genetics : EJHG [Eur J Hum Genet] 2022 Aug; Vol. 30 (8), pp. 922-929. Date of Electronic Publication: 2022 Mar 23.
DOI: 10.1038/s41431-022-01070-5
Abstrakt: The human leucocyte antigen (HLA) loci have been widely characterized to be associated with viral infectious diseases using either HLA allele frequency-based association or in silico predicted studies. However, there is less experimental evidence to link the HLA alleles with COVID-19 and other respiratory infectious diseases, particularly in the lung cells. To examine the role of HLA alleles in response to coronavirus and other respiratory viral infections in disease-relevant cells, we designed a two-stage study by integrating publicly accessible RNA-seq data sets, and performed allelic expression (AE) analysis on heterozygous HLA genotypes. We discovered an increased AE pattern accompanied with overexpression of HLA-B gene in SARS-CoV-2-infected human lung epithelial cells. Analysis of independent data sets verified the respiratory virus-induced AE of HLA-B gene in lung cells and tissues. The results were further experimentally validated in cultured lung cells infected with SARS-CoV-2. We further uncovered that the antiviral cytokine IFNβ contribute to AE of the HLA-B gene in lung cells. Our analyses provide a new insight into allelic influence on the HLA expression in association with SARS-CoV-2 and other common viral infectious diseases.
(© 2022. The Author(s), under exclusive licence to European Society of Human Genetics.)
Databáze: MEDLINE
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