Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation.

Autor: Wang M; Yale University School of Medicine, 375 Congress Ave, LSOG 405B, New Haven, CT, 06519, USA., Silva T; Yale University School of Medicine, 375 Congress Ave, LSOG 405B, New Haven, CT, 06519, USA., Toothaker JM; Yale University School of Medicine, 375 Congress Ave, LSOG 405B, New Haven, CT, 06519, USA.; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, 15213, USA., McCourt BT; Yale University School of Medicine, 375 Congress Ave, LSOG 405B, New Haven, CT, 06519, USA.; Department of Pediatrics, Yale University, New Haven, CT, 06520, USA., Shugrue C; Yale University School of Medicine, 375 Congress Ave, LSOG 405B, New Haven, CT, 06519, USA.; Department of Internal Medicine, Yale University, New Haven, CT, 06520, USA., Desir G; Yale University School of Medicine, 375 Congress Ave, LSOG 405B, New Haven, CT, 06519, USA.; Department of Internal Medicine, Yale University, New Haven, CT, 06520, USA.; VA CT Medical Center, Yale University, New Haven, CT, 06520, USA., Gorelick F; Yale University School of Medicine, 375 Congress Ave, LSOG 405B, New Haven, CT, 06519, USA.; Department of Internal Medicine, Yale University, New Haven, CT, 06520, USA.; VA CT Medical Center, Yale University, New Haven, CT, 06520, USA.; Department of Cell Biology, Yale University, New Haven, CT, 06520, USA., Konnikova L; Yale University School of Medicine, 375 Congress Ave, LSOG 405B, New Haven, CT, 06519, USA. liza.konnikova@yale.edu.; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, 15213, USA. liza.konnikova@yale.edu.; Department of Pediatrics, Yale University, New Haven, CT, 06520, USA. liza.konnikova@yale.edu.; Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University, New Haven, CT, 06520, USA. liza.konnikova@yale.edu.; Program in Human and Translational Immunology, Yale University, New Haven, CT, 06520, USA. liza.konnikova@yale.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Mar 23; Vol. 12 (1), pp. 4953. Date of Electronic Publication: 2022 Mar 23.
DOI: 10.1038/s41598-022-08817-6
Abstrakt: Placental function requires organized growth, transmission of nutrients, and an anti-inflammatory milieu between the maternal and fetal interface, but placental factors important for its function remain unclear. Renalase is a pro-survival, anti-inflammatory flavoprotein found to be critical in other tissues. We examined the potential role of renalase in placental development. PCR, bulk RNA sequencing, immunohistochemistry, and immunofluorescence for renalase and its binding partners, PMCA4b and PZP, were performed on human placental tissue from second-trimester and full-term placentas separated into decidua, placental villi and chorionic plates. Quantification of immunohistochemistry was used to localize renalase across time course from 17 weeks to term. Endogenous production of renalase was examined in placental tissue and organoids. Renalase and its receptor PMCA4b transcripts and proteins were present in all layers of the placenta. Estimated RNLS protein levels did not change with gestation in the decidual samples. However, placental villi contained more renalase immunoreactive cells in fetal than full-term placental samples. RNLS co-labeled with markers for Hofbauer cells and trophoblasts within the placental villi. Endogenous production of RNLS, PMCA4b, and PZP by trophoblasts was validated in placental organoids. Renalase is endogenously expressed throughout placental tissue and specifically within Hofbauer cells and trophoblasts, suggesting a potential role for renalase in placental development and function. Future studies should assess renalase's role in normal and diseased human placenta.
(© 2022. The Author(s).)
Databáze: MEDLINE
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