In vitro culture of ovine embryos up to early gastrulating stages.
| Autor: | Ramos-Ibeas P; Animal Reproduction Department, INIA-CSIC, Madrid 28040, Spain., González-Brusi L; Animal Reproduction Department, INIA-CSIC, Madrid 28040, Spain., Used MT; Animal Reproduction Department, INIA-CSIC, Madrid 28040, Spain., Cocero MJ; Animal Reproduction Department, INIA-CSIC, Madrid 28040, Spain., Marigorta P; Animal Reproduction Department, INIA-CSIC, Madrid 28040, Spain., Alberio R; School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, UK., Bermejo-Álvarez P; Animal Reproduction Department, INIA-CSIC, Madrid 28040, Spain. |
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| Jazyk: | angličtina |
| Zdroj: | Development (Cambridge, England) [Development] 2022 Mar 15; Vol. 149 (6). Date of Electronic Publication: 2022 Mar 23. |
| DOI: | 10.1242/dev.199743 |
| Abstrakt: | Developmental failures occurring shortly after blastocyst hatching from the zona pellucida constitute a major cause of pregnancy losses in both humans and farm ungulates. The developmental events occurring following hatching in ungulates include the proliferation and maturation of extra-embryonic membranes - trophoblast and hypoblast - and the formation of a flat embryonic disc, similar to that found in humans, which initiates gastrulation prior to implantation. Unfortunately, our understanding of these key processes for embryo survival is limited because current culture systems cannot sustain ungulate embryo development beyond hatching. Here, we report a culture system that recapitulates most developmental landmarks of gastrulating ovine embryos: trophoblast maturation, hypoblast migration, embryonic disc formation, disappearance of the Rauber's layer, epiblast polarization and mesoderm differentiation. Our system represents a highly valuable platform for exploring the cell differentiation, proliferation and migration processes governing gastrulation in a flat embryonic disc and for understanding pregnancy failures during the second week of gestation. This article has an associated 'The people behind the papers' interview. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.) |
| Databáze: | MEDLINE |
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