Shared signatures and divergence in skin microbiomes of children with atopic dermatitis and their caregivers.

Autor: Chia M; Genome Institute of Singapore and Agency for Science, Technology and Research (A∗STAR)., Naim ANM; Genome Institute of Singapore and Agency for Science, Technology and Research (A∗STAR)., Tay ASL; Skin Research Institute of Singapore, Agency for Science, Technology and Research (A∗STAR)., Lim K; Genome Institute of Singapore and Agency for Science, Technology and Research (A∗STAR)., Chew KL; Department of Laboratory Medicine, National University Health System, Singapore., Yow SJ; Infectious Diseases Programme and Department of Microbiology and Immunology., Chen J; Infectious Diseases Programme and Department of Microbiology and Immunology., Common JEA; Skin Research Institute of Singapore, Agency for Science, Technology and Research (A∗STAR). Electronic address: john_common@asrl.a-star.edu.sg., Nagarajan N; Genome Institute of Singapore and Agency for Science, Technology and Research (A∗STAR); Yong Loo Lin School of Medicine, National University of Singapore. Electronic address: nagarajann@gis.a-star.edu.sg., Tham EH; Yong Loo Lin School of Medicine, National University of Singapore; Khoo Teck Puat-National University Children's Medical Institute, National University Health System, Singapore. Electronic address: elizabeth_tham@nuhs.edu.sg.
Jazyk: angličtina
Zdroj: The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2022 Oct; Vol. 150 (4), pp. 894-908. Date of Electronic Publication: 2022 Mar 19.
DOI: 10.1016/j.jaci.2022.01.031
Abstrakt: Background: Atopic dermatitis (AD) is a common chronic skin condition in children (15-20%) that can significantly impair their quality of life. As a result of its relapsing nature and enrichment of Staphylococcus aureus during flares, clinical management can include eradicating S aureus from the skin of children; however, this does not extend to their healthy caregivers, who are potential reservoirs.
Objective: Our aim was to understand skin microbiome sharing and microbial features in children with AD and their healthy adult caregivers.
Methods: We utilized whole-metagenome profiling at 4 body sites (volar forearm, antecubital fossae, cheeks, and lesions) in combination with sequencing of S aureus isolates to characterize a cohort of children with AD and their healthy caregivers (n = 30 families) compared to matched pairs from control households (n = 30 families).
Results: Metagenomic analysis revealed distinct microbiome configurations in the nonlesional skin of AD children and their healthy caregivers versus controls, which were sufficient to accurately predict case-control status (area under the receiver operating characteristic curve > 0.8). These differences were accompanied by significant microbiome similarity between children and their caregivers, indicating that microbiome sharing may play a role in recurrent disease flares. Whole-genome comparisons with high-quality S aureus isolate genomes (n = 55) confirmed significant strain sharing between AD children and their caregivers and AD-specific enrichment of strains expressing enterotoxins Q and K/K2.
Conclusion: Our results highlight the distinctive skin microbiome features of healthy caregivers for children with AD and support their inclusion in strategies for the treatment of recurrent pediatric AD.
(Copyright © 2022 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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