DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy.

Autor: Yang B; Chan Zuckerberg Biohub, San Francisco, CA, USA. bin.yang@czbiohub.org., Lange M; Chan Zuckerberg Biohub, San Francisco, CA, USA., Millett-Sikking A; Calico Life Sciences LLC, South San Francisco, CA, USA., Zhao X; Chan Zuckerberg Biohub, San Francisco, CA, USA., Bragantini J; Chan Zuckerberg Biohub, San Francisco, CA, USA., VijayKumar S; Chan Zuckerberg Biohub, San Francisco, CA, USA., Kamb M; Chan Zuckerberg Biohub, San Francisco, CA, USA., Gómez-Sjöberg R; Chan Zuckerberg Biohub, San Francisco, CA, USA., Solak AC; Chan Zuckerberg Biohub, San Francisco, CA, USA., Wang W; Cardiovascular Research Institute, University of California, San Francisco (UCSF), San Francisco, CA, USA., Kobayashi H; Chan Zuckerberg Biohub, San Francisco, CA, USA., McCarroll MN; Department of Pharmaceutical Chemistry, University of California, San Francisco (UCSF), San Francisco, CA, USA., Whitehead LW; The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia., Fiolka RP; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.; Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX, USA., Kornberg TB; Cardiovascular Research Institute, University of California, San Francisco (UCSF), San Francisco, CA, USA., York AG; Calico Life Sciences LLC, South San Francisco, CA, USA., Royer LA; Chan Zuckerberg Biohub, San Francisco, CA, USA. loic.royer@czbiohub.org.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2022 Apr; Vol. 19 (4), pp. 461-469. Date of Electronic Publication: 2022 Mar 21.
DOI: 10.1038/s41592-022-01417-2
Abstrakt: The promise of single-objective light-sheet microscopy is to combine the convenience of standard single-objective microscopes with the speed, coverage, resolution and gentleness of light-sheet microscopes. We present DaXi, a single-objective light-sheet microscope design based on oblique plane illumination that achieves: (1) a wider field of view and high-resolution imaging via a custom remote focusing objective; (2) fast volumetric imaging over larger volumes without compromising image quality or necessitating tiled acquisition; (3) fuller image coverage for large samples via multi-view imaging and (4) higher throughput multi-well imaging via remote coverslip placement. Our instrument achieves a resolution of 450 nm laterally and 2 μm axially over an imaging volume of 3,000 × 800 × 300 μm. We demonstrate the speed, field of view, resolution and versatility of our instrument by imaging various systems, including Drosophila egg chamber development, zebrafish whole-brain activity and zebrafish embryonic development - up to nine embryos at a time.
(© 2022. The Author(s).)
Databáze: MEDLINE
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