Orally active bivalent V H H construct prevents proliferation of F4 + enterotoxigenic Escherichia coli in weaned piglets.

Autor: Fiil BK; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark., Thrane SW; Bactolife ApS, Lersø Parkallé 38, 2100 Copenhagen East, Denmark., Pichler M; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark., Kittilä T; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark., Ledsgaard L; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark., Ahmadi S; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark., Hermansen GMM; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark.; Bactolife ApS, Lersø Parkallé 38, 2100 Copenhagen East, Denmark., Jelsbak L; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark., Lauridsen C; Department of Animal Science, Aarhus University, Blichers Alle 20, 8830 Tjele, Denmark., Brix S; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark., Laustsen AH; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark.; Bactolife ApS, Lersø Parkallé 38, 2100 Copenhagen East, Denmark.
Jazyk: angličtina
Zdroj: IScience [iScience] 2022 Mar 01; Vol. 25 (4), pp. 104003. Date of Electronic Publication: 2022 Mar 01 (Print Publication: 2022).
DOI: 10.1016/j.isci.2022.104003
Abstrakt: A major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) in piglets, often caused by enterotoxigenic Escherichia coli (ETEC). The increased use of antibiotics and zinc oxide to treat PWD has raised global concerns regarding antimicrobial resistance development and environmental pollution. Still, alternative treatments targeting ETEC and counteracting PWD are largely lacking. Here, we report the design of a pH, temperature, and protease-stable bivalent V H H-based protein BL1.2 that cross-links a F4 + ETEC model strain by selectively binding to its fimbriae. This protein inhibits F4 + ETEC adhesion to porcine epithelial cells ex vivo and decreases F4 + ETEC proliferation when administrated as a feed additive to weaned F4 + ETEC challenged piglets. These findings highlight the potential of a highly specific bivalent V H H-based feed additive in effectively delimiting pathogenic F4 + ETEC bacteria proliferation in piglets and may represent a sustainable solution for managing PWD while circumventing antimicrobial resistance development.
Competing Interests: SWT and AHL are co-founders, employees, and shareholders in Bactolife ApS, and they are inventors behind the patent application WO2020144164A1. The authors declare no other conflicts of interest.
(© 2022 The Author(s).)
Databáze: MEDLINE
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