Complete Genome Assemblies of All Xanthomonas translucens Pathotype Strains Reveal Three Genetically Distinct Clades.

Autor:	Goettelmann F; Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zürich, Zurich, Switzerland., Roman-Reyna V; Department of Plant Pathology, The Ohio State University, Columbus, OH, United States.; Infectious Diseases Institute, The Ohio State University, Columbus, OH, United States., Cunnac S; Plant Health Institute of Montpellier, University of Montpellier, CIRAD, INRAE, Institut Agro, IRD, Montpellier, France., Jacobs JM; Department of Plant Pathology, The Ohio State University, Columbus, OH, United States.; Infectious Diseases Institute, The Ohio State University, Columbus, OH, United States., Bragard C; Earth and Life Institute, UCLouvain, Louvain-la-Neuve, Belgium., Studer B; Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zürich, Zurich, Switzerland., Koebnik R; Plant Health Institute of Montpellier, University of Montpellier, CIRAD, INRAE, Institut Agro, IRD, Montpellier, France., Kölliker R; Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zürich, Zurich, Switzerland.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2022 Mar 02; Vol. 12, pp. 817815. Date of Electronic Publication: 2022 Mar 02 (Print Publication: 2021).
DOI:	10.3389/fmicb.2021.817815
Abstrakt:	The Xanthomonas translucens species comprises phytopathogenic bacteria that can cause serious damage to cereals and to forage grasses. So far, the genomic resources for X. translucens were limited, which hindered further understanding of the host-pathogen interactions at the molecular level and the development of disease-resistant cultivars. To this end, we complemented the available complete genome sequence of the X. translucens pv. translucens pathotype strain DSM 18974 by sequencing the genomes of all the other 10 X. translucens pathotype strains using PacBio long-read technology and assembled complete genome sequences. Phylogeny based on average nucleotide identity (ANI) revealed three distinct clades within the species, which we propose to classify as clades Xt-I, Xt-II, and Xt-III. In addition to 2,181 core X. translucens genes, a total of 190, 588, and 168 genes were found to be exclusive to each clade, respectively. Moreover, 29 non-transcription activator-like effector (TALE) and 21 TALE type III effector classes were found, and clade- or strain-specific effectors were identified. Further investigation of these genes could help to identify genes that are critically involved in pathogenicity and/or host adaptation, setting the grounds for the development of new resistant cultivars. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Goettelmann, Roman-Reyna, Cunnac, Jacobs, Bragard, Studer, Koebnik and Kölliker.)
Databáze:	MEDLINE
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