Persistent SARS-CoV-2 antigen presence in multiple organs of a naturally infected cat from Brazil.

Autor:	Jarrah SA; Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil., Kmetiuk LB; Graduate College of Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil., de Carvalho OV; Graduate College of Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil., de Sousa ATHI; Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil., Souza VRF; Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil., Nakazato L; Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil., Colodel EM; Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil., Dos Santos AP; Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA., Pettan-Brewer C; Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, Washington, USA., Hahn RC; Medical Science Faculty, Federal University of Mato Grosso, Cuiabá, MT, Brazil., Slhessarenko RD; Júlio Muller University Hospital, Federal University of Mato Grosso, Cuiabá, MT, Brazil., Ubiali DG; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropedica, RJ, Brazil., Pereira AHB; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropedica, RJ, Brazil., de Morais HA; Department of Clinical Sciences, Oregon State University, Corvallis, OR, United States., Biondo AW; Department of Veterinary Medicine, Federal University of Paraná, Curitiba, PR, Brazil., Dutra V; Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil.
Jazyk:	angličtina
Zdroj:	The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2022 Mar 07; Vol. 28, pp. e20210074. Date of Electronic Publication: 2022 Mar 07 (Print Publication: 2022).
DOI:	10.1590/1678-9199-JVATITD-2021-0074
Abstrakt:	Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent of the disease coronavirus 2019 (COVID-19) in humans. SARS-CoV-2 has been identified in cats with or without clinical signs. Case Presentation: We describe the pathological and molecular findings in a six-month-old asymptomatic cat with SARS-CoV-2 infection from Brazil, belonging to a human family with COVID-19 cases. The pool of nasopharynx and oropharynx swabs at day zero tested positive by RT-qPCR for SARS-CoV-2. No amplification resulted from molecular testing performed on days 7 and 14. The cat was hit by a car and died 43 days after the molecular diagnosis. Immunohistochemistry at post-mortem examination demonstrated nucleocapsid protein in samples from the lungs, kidneys, nasal conchae, trachea, intestine, brain and spleen. Conclusion: The present study has highlighted the possibility that viral antigens can be detected by immunohistochemistry in multiple organs six weeks after infection, although the same tissues tested negative by RT-PCR. Competing Interests: Competing interests: The authors declare that they have no competing interests.
Databáze:	MEDLINE
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