Expression and 7-day time course of circulating microRNAs in septic patients treated with nephrotoxic antibiotic agents.

Autor: Petejova N; Department of Internal Medicine and Cardiology, University Hospital Ostrava, 70852, Ostrava, Czech Republic. petejova@seznam.cz.; Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic. petejova@seznam.cz.; Department of Internal Medicine III-Nephrology, Rheumatology and Endocrinology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77900, Olomouc, Czech Republic. petejova@seznam.cz., Martinek A; Department of Internal Medicine and Cardiology, University Hospital Ostrava, 70852, Ostrava, Czech Republic.; Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic., Zadrazil J; Department of Internal Medicine III-Nephrology, Rheumatology and Endocrinology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77900, Olomouc, Czech Republic.; Department of Internal Medicine III-Nephrology, Rheumatology and Endocrinology, University Hospital Olomouc, 77900, Olomouc, Czech Republic., Klementa V; Department of Internal Medicine III-Nephrology, Rheumatology and Endocrinology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 77900, Olomouc, Czech Republic.; Department of Internal Medicine III-Nephrology, Rheumatology and Endocrinology, University Hospital Olomouc, 77900, Olomouc, Czech Republic., Pribylova L; Department of Applied Mathematics, Faculty of Electrical Engineering and Computer Science, VSB Technical University of Ostrava, 70800, Ostrava, Czech Republic., Bris R; Department of Applied Mathematics, Faculty of Electrical Engineering and Computer Science, VSB Technical University of Ostrava, 70800, Ostrava, Czech Republic., Kanova M; Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic.; Department of Anesthesiology and Resuscitation, University Hospital Ostrava, 70852, Ostrava, Czech Republic., Sigutova R; Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic.; Department of Epidemiology and Public Health, Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic.; Department of Laboratory Diagnostics Institute of Clinical Biochemistry and Clinical Pharmacology, University Hospital Ostrava, 70852, Ostrava, Czech Republic., Kacirova I; Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic.; Department of Laboratory Diagnostics Institute of Clinical Biochemistry and Clinical Pharmacology, University Hospital Ostrava, 70852, Ostrava, Czech Republic., Svagera Z; Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic.; Department of Laboratory Diagnostics Institute of Clinical Biochemistry and Clinical Pharmacology, University Hospital Ostrava, 70852, Ostrava, Czech Republic., Bace E; Department of Immunodiagnostics, BioVendor R&D, Laboratory Medicine Corp, 621 00, Brno, Czech Republic., Stejskal D; Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic.; Department of Laboratory Diagnostics Institute of Clinical Biochemistry and Clinical Pharmacology, University Hospital Ostrava, 70852, Ostrava, Czech Republic.
Jazyk: angličtina
Zdroj: BMC nephrology [BMC Nephrol] 2022 Mar 19; Vol. 23 (1), pp. 111. Date of Electronic Publication: 2022 Mar 19.
DOI: 10.1186/s12882-022-02726-6
Abstrakt: Background: Through regulation of signaling pathways, microRNAs (miRNAs) can be involved in sepsis and associated organ dysfunction. The aims of this study were to track the 7-day time course of blood miRNAs in patients with sepsis treated with vancomycin, gentamicin, or a non-nephrotoxic antibiotic and miRNA associations with neutrophil gelatinase-associated lipokalin (NGAL), creatinine, procalcitonin, interleukin-6, and acute kidney injury (AKI) stage.
Methods: Of 46 adult patients, 7 were on vancomycin, 20 on gentamicin, and 19 on another antibiotic. Blood samples were collected on days 1, 4, and 7 of treatment, and miRNAs were identified using quantitative reverse transcription PCR.
Results: The results showed no relationship between miRNA levels and biochemical variables on day 1. By day 7 of gentamicin treatment miR-15a-5p provided good discrimination between AKI and non-AKI (area under curve, 0.828). In patients taking vancomycin, miR-155-5p and miR-192-5p positively correlated with creatinine and NGAL values, and miR-192-5p and miR-423-5p positively correlated with procalcitonin and interleukin-6 in patients treated with a non-nephrotoxic antibiotic. In patients together we found positive correlation between miR-155-5p and miR-423-5p and all biochemical markers.
Conclusion: The results suggest that these four miRNAs may serve as diagnostic or therapeutic tool in sepsis, renal injury and nephrotoxic treatment.
Trial Registration: ClinicalTrials.gov , ID: NCT04991376 . Registered on 27 July 2021.
(© 2022. The Author(s).)
Databáze: MEDLINE
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