Evolutionary divergence of Firre localization and expression.

Autor:	Much C; BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado 80303, USA., Smallegan MJ; BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado 80303, USA.; Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, Colorado 80302, USA., Hwang T; BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado 80303, USA., Hanson SD; BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado 80303, USA., Dumbović G; BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado 80303, USA.; Institute for Cardiovascular Regeneration, Goethe University Frankfurt, 60596 Frankfurt am Main, Germany., Rinn JL; BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado 80303, USA.; Department of Biochemistry, University of Colorado Boulder, Boulder, Colorado 80302, USA.
Jazyk:	angličtina
Zdroj:	RNA (New York, N.Y.) [RNA] 2022 Jun; Vol. 28 (6), pp. 842-853. Date of Electronic Publication: 2022 Mar 18.
DOI:	10.1261/rna.079070.121
Abstrakt:	Long noncoding RNAs (lncRNAs) are rapidly evolving and thus typically poorly conserved in their sequences. How these sequence differences affect the characteristics and potential functions of lncRNAs with shared synteny remains unclear. Here we show that the syntenically conserved lncRNA Firre displays distinct expression and localization patterns in human and mouse. Single molecule RNA FISH reveals that in a range of cell lines, mouse Firre ( mFirre ) is predominantly nuclear, while human FIRRE ( hFIRRE ) is distributed between the cytoplasm and nucleus. This localization pattern is maintained in human/mouse hybrid cells expressing both human and mouse Firre , implying that the localization of the lncRNA is species autonomous. We find that the majority of hFIRRE transcripts in the cytoplasm are comprised of isoforms that are enriched in RRD repeats. We furthermore determine that in various tissues, mFirre is more highly expressed than its human counterpart. Our data illustrate that the rapid evolution of syntenic lncRNAs can lead to variations in lncRNA localization and abundance, which in turn may result in disparate lncRNA functions even in closely related species. (© 2022 Much et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)
Databáze:	MEDLINE
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