Sublethal doses of imidacloprid and pyraclostrobin impair fat body of solitary bee Tetrapedia diversipes (Klug, 1810).

Autor: Conceição de Assis J; Federal University of São Carlos, UFSCar, Postgraduate Program in Biotechnology and Environmental Monitoring, Sorocaba, São Paulo State, Brazil., Eduardo da Costa Domingues C; University of Maribor, Faculty of Agriculture and Life Sciences, Pivola, Slovenia., Tadei R; São Paulo State University, UNESP, Postgraduate Program in Biological Sciences, Rio Claro, São Paulo State, Brazil., Inês da Silva C; Federal University of São Carlos, UFSCar, Department of Biology, Laboratory of Ecotoxicology and Environmental Integrity (LEIA), Sorocaba, São Paulo State, Brazil; Federal University of São Carlos, UFSCar, Department of Environmental Sciences, Sorocaba, São Paulo State, Brazil., Soares Lima HM; Federal University of São Carlos, UFSCar, Department of Biology, Laboratory of Ecotoxicology and Environmental Integrity (LEIA), Sorocaba, São Paulo State, Brazil., Decio P; Federal University of São Carlos, UFSCar, Postgraduate Program in Biotechnology and Environmental Monitoring, Sorocaba, São Paulo State, Brazil; Federal University of São Carlos, UFSCar, Department of Biology, Laboratory of Ecotoxicology and Environmental Integrity (LEIA), Sorocaba, São Paulo State, Brazil., Silva-Zacarin ECM; Federal University of São Carlos, UFSCar, Postgraduate Program in Biotechnology and Environmental Monitoring, Sorocaba, São Paulo State, Brazil; Federal University of São Carlos, UFSCar, Department of Biology, Laboratory of Ecotoxicology and Environmental Integrity (LEIA), Sorocaba, São Paulo State, Brazil. Electronic address: elaine@ufscar.br.
Jazyk: angličtina
Zdroj: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2022 Jul 01; Vol. 304, pp. 119140. Date of Electronic Publication: 2022 Mar 14.
DOI: 10.1016/j.envpol.2022.119140
Abstrakt: Solitary bees present greater species diversity than social bees. However, they are less studied than managed bees, mainly regarding the harmful effects of pesticides present in agroecosystems commonly visited by them. This study aimed to evaluate the effect of residual doses of imidacloprid and pyraclostrobin, alone and in combination, on the fat body (a multifunctional organ) of the neotropical solitary bee Tetrapedia diversipes by means of morphological and histochemical evaluation of oenocytes and trophocytes. Males and females of newly-emerged adults were submitted to bioassays of acute topical exposure. Experimental groups were essayed: control (CTR), solvent control (ACT), imidacloprid (IMI, 0.0028 ng/μL), pyraclostrobin (PYR, 2.7 ng/μL) and imidacloprid + pyraclostrobin (I + P). The data demonstrated that the residual doses applied in T. diversipes adults are sublethal at 96 h. Both oenocytes and trophocytes cells responded to topical exposure to the pesticides, showing morphological changes. In the IMI group, the bee oenocytes showed the greatest proportion of vacuolization and altered nuclei. The pyraclostrobin exposure increased the intensity of PAS-positive labeling (glycogen) in trophocytes. This increase was also observed in the I + P group. Changes in energy reserve (glycogen) of trophocytes indicate a possible mobilization impairment of this neutral polysaccharide to the hemolymph, which can compromise the fitness of exposed individuals. Also, changes in oenocytes can compromise the detoxification function performed by the fat body. This is the first study to show sublethal effects in neotropical solitary bees and highlight the importance of studies with native bees.
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Databáze: MEDLINE
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