Natural COA water inhibits mitochondrial ROS-mediated apoptosis through Plk3 downregulation under STZ diabetic stress in pancreatic β-cell lines.

Autor:	Lee J; Division of Radiology, Mayo Clinic, Rochester, MN, 55905, USA., Chung JO; Department of Internal Medicine, Chonnam National University Medical School, Gwangju, 501757, Republic of Korea., Park SY; Department of Internal Medicine, Chonnam National University Medical School, Gwangju, 501757, Republic of Korea., Rajamohan N; Division of Radiology, Mayo Clinic, Rochester, MN, 55905, USA., Singh A; Division of Radiology, Mayo Clinic, Rochester, MN, 55905, USA., Kim J; Division of Radiology, Mayo Clinic, Rochester, MN, 55905, USA.; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, MN, 55905, USA., Lowe VJ; Division of Radiology, Mayo Clinic, Rochester, MN, 55905, USA., Lee S; Division of Radiology, Mayo Clinic, Rochester, MN, 55905, USA.; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, MN, 55905, USA.
Jazyk:	angličtina
Zdroj:	Biochemistry and biophysics reports [Biochem Biophys Rep] 2022 Mar 11; Vol. 30, pp. 101247. Date of Electronic Publication: 2022 Mar 11 (Print Publication: 2022).
DOI:	10.1016/j.bbrep.2022.101247
Abstrakt:	Diabetes from pancreatic β cell death and insulin resistance is a serious metabolic disease in the world. Although the overproduction of mitochondrial reactive oxygen species (ROS) plays an important role in the pathogenesis of diabetes, its specific molecular mechanism remains unclear. Here, we show that the natural Charisma of Aqua (COA) water plays a role in Streptozotocin (STZ) diabetic stress-induced cell death inhibition. STZ induces mitochondrial ROS by increasing Polo-like kinase 3 (Plk3), a major mitotic regulator, in both Beta TC-6 and Beta TC-tet mouse islet cells and leads to apoptosis. Overexpression of Plk3 regulates an increase in mitochondrial ROS as well as cell death, also these events were inhibited by Plk3 gene knockdown in STZ diabetic stimulated-Beta TC-6 cells. Interestingly, we found that natural COA water blocks mitochondrial ROS generation through the reduction of Plk3 and prevents apoptosis in STZ-treated beta cells. Furthermore, using the 3D organoid ( ex vivo ) system, we confirmed that the insulin secretion of the supernatant medium under STZ treated pancreatic β-cells is protected by the natural COA water. These findings demonstrate that the natural water COA has a beneficial role in maintaining β cell function through the inhibition of mitochondrial ROS-mediated cell death, and it might be introduced as a potential insulin stabilizer. Competing Interests: Dr. Lowe is a consultant for AVID Radiopharmaceuticals, Eisai Co. Inc., Bayer Schering Pharma, GE Healthcare, and Merck Research, and receives research support from GE Healthcare, Siemens Molecular Imaging, AVID Radiopharmaceuticals, and NIH (NIA,NCI). Dr. Lee report grants from E&P Co., Ltd. (South Korea) during the conduct of the study. (© 2022 The Authors.)
Databáze:	MEDLINE
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