Activation-induced colocalisation of SCAMP5 with IFNα in human plasmacytoid dendritic cells.

Autor:	Pouw JN; Rheumatology and Clinical Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands j.n.pouw-3@umcutrecht.nl.; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands., Olde Nordkamp MAM; Rheumatology and Clinical Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands.; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands., O'Toole TG; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands., Radstake TRDJ; Rheumatology and Clinical Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands.; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands., Leijten EFA; Rheumatology and Clinical Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands.; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands.; Rheumatology, Sint Maartenskliniek, Nijmegen, The Netherlands., Boes M; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands.; Pediatric Immunology, Wilhelmina Children's Hospital University Medical Centre, Utrecht University, Utrecht, The Netherlands.
Jazyk:	angličtina
Zdroj:	Lupus science & medicine [Lupus Sci Med] 2022 Mar; Vol. 9 (1).
DOI:	10.1136/lupus-2022-000680
Abstrakt:	Introduction: Plasmacytoid dendritic cells (pDCs) are the main producers of type I interferon (IFN) in SLE. pDCs express high secretory carrier membrane protein 5 (SCAMP5). Recent work in transfected HEK cells connects SCAMP5 to the type I IFN secretory pathway. To further study the role of SCAMP5 in IFNα secretion by pDCs, we focused on the subcellular distribution of SCAMP5 in human pDCs freshly isolated from peripheral blood. Methods: We measured SCAMP5 expression by flow cytometry in peripheral blood mononuclear cells of healthy subjects (n=8). Next, we assessed the colocalisation of SCAMP5 with IFNα in pDCs of healthy subjects (n=4) by evaluating bright detail similarity (BDS) scores using ImageStream technology. Results: We confirm that SCAMP5 is highly expressed by pDCs derived from peripheral blood. In activated pDCs, we show that SCAMP5 colocalises with IFNα (mean BDS 2.0±0.1; BDS >2.0 in 44% of pDCs). Conclusion: SCAMP5 colocalises with IFNα in activated human pDCs, in support of a role of this trafficking protein in the secretion of type I IFN by pDCs. Competing Interests: Competing interests: MB reports research grants from Actuate Therapeutics, Nutricia and Argenx, unrelated to the submitted work. All other authors state no conflict of interest and have no disclosures. (© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
Databáze:	MEDLINE
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