Molecular characterization of picobirnaviruses in small ruminants with diarrhea in Turkey.

Autor: Karayel-Hacioglu I; Department of Virology, Faculty of Veterinary Medicine, Ankara University, Diskapi, 06110, Ankara, Turkey. ilkeekarayel@gmail.com., Gul B; Department of Virology, Graduate School of Health Sciences, Ankara University, Sehit Omer Halisdemir Street, Diskapi, 06110, Ankara, Turkey., Alkan F; Department of Virology, Faculty of Veterinary Medicine, Ankara University, Diskapi, 06110, Ankara, Turkey.
Jazyk: angličtina
Zdroj: Virus genes [Virus Genes] 2022 Jun; Vol. 58 (3), pp. 238-243. Date of Electronic Publication: 2022 Mar 15.
DOI: 10.1007/s11262-022-01894-9
Abstrakt: Picobirnaviruses (PBVs), detected in a wide range of host species, are viruses of which limited information is available about their pathogenic potential, ecology, or evolutionary characteristics. In this study, a molecular analysis of segment 2 encoding the PBV RNA-dependent RNA-polymerase (RdRp) in small ruminants with diarrhea in Turkey was undertaken. A total of 66 fecal samples or gut contents from diarrheic small ruminants including 55 sheep and 11 goats were screened. Four samples (6.06%), obtained from sheep in different farms, yielded the expected amplicon size for the genogroup I RdRp gene fragment, whereas no positivity was detected for genogroup II PBVs. Phylogenetic analysis revealed high levels of genetic diversity among the genogroup I PBVs. Additionally, all PBV infected sheep were also positive for rotavirus A. This study, reporting the presence of the PBVs in sheep Turkey for the first time, contributes to the molecular characterization and epidemiology of PBVs.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: