Methylation at CpG sites related to growth differentiation factor-15 was not prospectively associated with cardiovascular death in discordant monozygotic twins.

Autor: Moore SS; Doctoral Program of Osteopathic Medicine, College of Osteopathic Medicine, Des Moines University, Des Moines, IA, 50312, USA., Mukherji P; Emergency Medicine Residency Program, Loyola University Medical Center, Maywood, IL, 60153, USA., Leung M; Institute for Personalized Medicine, Penn State College of Medicine, Hershey, PA, 17033, USA., Vrentas CE; Master Program of Public Health, Des Moines University, Des Moines, IA, 50312, USA., Mwanja MM; Oregon State Department of Human Services, Salem, OR, 97303, USA., Dai J; Department of Public Health, College of Health Sciences, Des Moines University, 3200 Grand Avenue, Des Moines, IA, 50312, USA. Jun.Dai@dmu.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Mar 15; Vol. 12 (1), pp. 4410. Date of Electronic Publication: 2022 Mar 15.
DOI: 10.1038/s41598-022-08369-9
Abstrakt: Myocardial infarction patients had decreased methylation at four growth differentiating factor-15 (GDF-15) related CpG sites (cg13033858, cg16936953, cg17150809, and cg18608055). These sites had not been studied for their association with cardiovascular disease (CVD) deaths. Thus, we aimed to assess the associations independent of genes, shared environment, and traditional CVD risk factors. Nineteen white, male, monozygotic twin pairs discordant for CVD deaths were included from the National Heart, Lung and Blood Institute Twin Study (NHLBI) initiated in 1969. Data on vital status was collected through December 31, 2014. Methylation of buffy coat DNA at exam 3 (1986-87) was measured using the Illumina HumanMethylation450 BeadChip. Principal component analysis was used to generate a score representing blood leukocyte composition and baseline CVD risk factors and predominated with natural killer cells, CD4+ T cells, and Framingham risk score. Conditional logistic regression demonstrated that methylation at the four CpG sites was not associated with CVD deaths before (all p > 0.05, bootstrapped p > 0.05) and after adjustment for the score (all p > 0.05). Joint influences of cg16936953 and the score were statistically significant (p < 0.05). In conclusion, joint influences of methylation at the site cg16936953 and the score are prospectively associated with CVD deaths independent of germline and common environment.ClinicalTrials.gov Identifier for NHLBI Twin Study: NCT00005124.
(© 2022. The Author(s).)
Databáze: MEDLINE
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