Gastroprotective effect of hydroalcoholic extract from Agaricus blazei Murill against ethanol-induced gastric ulcer in mice.

Autor: Câmara Neto JF; Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil., Campelo MDS; Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil., Cerqueira GS; Núcleo de Ensino e Pesquisa em Microscopia e Processamento de Imagens, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil. Electronic address: giufarmacia@hotmail.com., de Miranda JAL; Núcleo de Ensino e Pesquisa em Microscopia e Processamento de Imagens, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil., Guedes JAC; Embrapa Agroindústria Tropical. Rua Dra. Sara Mesquita, 2270 - Pici, CEP 60020-181, Fortaleza, CE, Brasil., de Almeida RR; Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil., Soares SA; Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil., Gramosa NV; Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil., Zocolo GJ; Embrapa Agroindústria Tropical. Rua Dra. Sara Mesquita, 2270 - Pici, CEP 60020-181, Fortaleza, CE, Brasil., Vieira ÍGP; Parque de Desenvolvimento Tecnológico, Universidade Federal do Ceará, Avenida do Contorno, CEP 60455-970, Fortaleza, CE, Brasil., Ricardo NMPS; Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil. Electronic address: naricard@ufc.br., Ribeiro MENP; Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, CEP 60440-900, Brasil. Electronic address: elenir.ribeiro@ufc.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2022 Jun 28; Vol. 292, pp. 115191. Date of Electronic Publication: 2022 Mar 12.
DOI: 10.1016/j.jep.2022.115191
Abstrakt: Ethnopharmacological Relevance: The use of mushrooms in medicine is quite old and the first report about the use of genus Agaricus in treatment of ulcers occurred in Byzantine period. This mushroom is widely consumed as food, tea, food supplements, as well as nutraceutical and cosmeceutical applications, being cultivated and appreciated in several countries such as Brazil, Korea, Japan and China.
Aim of the Study: This study aimed to characterize the chemical profile and the potential gastroprotective effect of hydroalcoholic extract from Agaricus blazei Murill (HEAb).
Materials and Methods: The extract was chemically characterized by elemental analysis, UPLC-QTOF-MS E , Nuclear Magnetic Resonance (NMR) and high-performance liquid chromatography (HPLC) techniques to elucidate the metabolites present in the extract. The quantification of phenolic compounds and the in vitro antioxidant activities were performed and the gastroprotective effect of this extract was evaluated against ethanol-induced gastric ulcer model. HEAb was administered by gavage at 5, 25 and 50 mg kg -1 and N-acetylcysteine at 300 mg kg -1 (positive control). Furthermore, the pathways of nitric oxide (NO), Cyclic Guanylate Monophosphate (cGMP), prostaglandins (PGs) and the involvement of ATP-sensitive K + Channels were modulated.
Results: Mannitol, malic acid, pyroglutamic acid, L-agaritine and L-valine were putatively identified by UPLC-QTOF-MS E in HEAb. In addition, it was possible to identify mannitol by the intense signals in the NMR spectra, being still quantified as the main compound in the extract by HPLC. The contents of total phenols and flavonoids corroborated with the good antioxidant activity of HEAb. This study observed that HEAb at 25 and 50 mg kg -1 had gastroprotection effect demonstrated by the reduction of histopathological parameters and the reduction of mastocytosis in the stomach of mice.
Conclusions: In this study was possible to conclude that HEAb has gastroprotective effect related to the involvement of NO and PG pathways in the ethanol-induced gastric ulcer model in mice.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: