Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells.
| Autor: | Townley BA; Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA., Soll JM; Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA., Mosammaparast N; Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA. nima@wustl.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2444, pp. 29-41. |
| DOI: | 10.1007/978-1-0716-2063-2_3 |
| Abstrakt: | Immunoaffinity purification allows for the purification of epitope-tagged proteins and their associated multisubunit complexes from mammalian cells. Subsequent identification of the proteins by proteomic analysis enables unbiased biochemical characterization of their associated partners, potentially revealing the physiological or functional context of any given protein. Here, we use immunoaffinity isolation of the Activating Signal Co-integrator Complex (ASCC) from human cells as an example, demonstrating the utility of the approach in revealing protein complexes involved in genotoxic stress responses. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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