Detection of micro-RNA by a combination of nucleic acid sequence-based amplification and a novel chemiluminescent pyrophosphate assay.

Autor: Karasawa K; Division of Clinical Nutrition and Metabolism, Department of Clinical Pharmacy, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo, Japan., Arakawa H; Division of Clinical Nutrition and Metabolism, Department of Clinical Pharmacy, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo, Japan.
Jazyk: angličtina
Zdroj: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2022 May; Vol. 37 (5), pp. 822-827. Date of Electronic Publication: 2022 Mar 22.
DOI: 10.1002/bio.4226
Abstrakt: Micro-RNA has attracted much attention as a biomarker for disease progression and malignancy. A compact, simple, rapid, and highly sensitive method is required to perform simple genetic analyses, such as point-of-care testing (POCT), at the clinic or bedside. Nucleic acid sequence-based amplification (NASBA) is a specific amplification method for a single-stranded RNA fragment that is useful for the highly sensitive detection of miRNAs. In this work, we developed a novel miRNA analytical system for POCT by combining the NASBA and chemiluminescence methods. Because the NASBA reaction is conducted at a constant temperature (41°C) and detection by chemiluminescence reaction does not require a light source, these methods could be combined to amplify 100 ng/assay miRNA. This combined miRNA detection method could be useful for the future development of compact POCT systems.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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