The Inability of Ex Vivo Expanded Mesenchymal Stem/Stromal Cells to Survive in Newborn Mice and to Induce Transplantation Tolerance.

Autor: Holan V; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic. vladimir.holan@iem.cas.cz.; Department of Cell Biology, Faculty of Science, Charles University, 12843, Prague 2, Czech Republic. vladimir.holan@iem.cas.cz., Echalar B; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic.; Department of Cell Biology, Faculty of Science, Charles University, 12843, Prague 2, Czech Republic., Palacka K; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic.; Department of Cell Biology, Faculty of Science, Charles University, 12843, Prague 2, Czech Republic., Kossl J; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic.; Department of Cell Biology, Faculty of Science, Charles University, 12843, Prague 2, Czech Republic., Bohacova P; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic.; Department of Cell Biology, Faculty of Science, Charles University, 12843, Prague 2, Czech Republic., Porubská B; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic.; Department of Cell Biology, Faculty of Science, Charles University, 12843, Prague 2, Czech Republic., Krulova M; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic.; Department of Cell Biology, Faculty of Science, Charles University, 12843, Prague 2, Czech Republic., Javorkova E; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic.; Department of Cell Biology, Faculty of Science, Charles University, 12843, Prague 2, Czech Republic., Zajicova A; Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220, Prague 4, Czech Republic.
Jazyk: angličtina
Zdroj: Stem cell reviews and reports [Stem Cell Rev Rep] 2022 Oct; Vol. 18 (7), pp. 2365-2375. Date of Electronic Publication: 2022 Mar 15.
DOI: 10.1007/s12015-022-10363-7
Abstrakt: An encounter of the developing immune system with an antigen results in the induction of immunological areactivity to this antigen. In the case of transplantation antigens, the application of allogeneic hematopoietic cells induces a state of neonatal transplantation tolerance. This tolerance depends on the establishment of cellular chimerism, when allogeneic cells survive in the neonatally treated recipient. Since mesenchymal stem/stromal cells (MSCs) have been shown to have low immunogenicity and often survive in allogeneic recipients, we attempted to use these cells for induction of transplantation tolerance. Newborn (less than 24 h old) C57BL/6 mice were injected intraperitoneally with 5 × 10 6 adipose tissue-derived MSCs isolated from allogeneic donors and the fate and survival of these cells were monitored. The impact of MSC application on the proportion of cell populations of the immune system and immunological reactivity was assessed. In addition, the survival of skin allografts in neonatally treated recipients was tested. We found that in vitro expanded MSCs did not survive in neonatal recipients, and the living MSCs were not detected few days after their application. Furthermore, there were no significant changes in the proportion of individual immune cell populations including CD4 + cell lineages, but we detected an apparent shift to the production of Th1 cytokines IL-2 and IFN-γ in neonatally treated mice. However, skin allografts in the MSC-treated recipients were promptly rejected. These results therefore show that in vitro expanded MSCs do not survive in neonatal recipients, but induce a cytokine imbalance without induction of transplantation tolerance.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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