HS1 deficiency protects against sepsis by attenuating neutrophil-inflicted lung damage.

Autor: Guerrero-Fonseca IM; Department of Molecular Biomedicine, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico., García-Ponce A; Department of Molecular Biomedicine, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico., Vadillo E; Department of Molecular Biomedicine, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico., Lartey NL; Department of Molecular Biomedicine, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico., Vargas-Robles H; Department of Molecular Biomedicine, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico., Chánez-Paredes S; Department of Molecular Biomedicine, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico., Castellanos-Martínez R; Department of Molecular Biomedicine, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico., Nava P; Department of Physiology, Biophysics and Neurosciences, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico., Betanzos A; Department of Infectomics and Molecular Pathogenesis, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico; Conacyt, Mexico City, Mexico., Neumann BM; Brown University Warren Alpert Medical School, Division of Surgical Research, Rhode Island Hospital, Providence, RI, USA., Penkala-Auguste K; Brown University Warren Alpert Medical School, Division of Surgical Research, Rhode Island Hospital, Providence, RI, USA., Lefort CT; Brown University Warren Alpert Medical School, Division of Surgical Research, Rhode Island Hospital, Providence, RI, USA., Schnoor M; Department of Molecular Biomedicine, CINVESTAV-IPN, Avenida IPN 2508, 07360 Mexico City, Mexico. Electronic address: mschnoor@cinvestav.mx.
Jazyk: angličtina
Zdroj: European journal of cell biology [Eur J Cell Biol] 2022 Apr; Vol. 101 (2), pp. 151214. Date of Electronic Publication: 2022 Mar 09.
DOI: 10.1016/j.ejcb.2022.151214
Abstrakt: Sepsis remains an important health problem worldwide due to inefficient treatments often resulting in multi-organ failure. Neutrophil recruitment is critical during sepsis. While neutrophils are required to combat invading bacteria, excessive neutrophil recruitment contributes to tissue damage due to their arsenal of molecular weapons that do not distinguish between host and pathogen. Thus, neutrophil recruitment needs to be fine-tuned to ensure bacterial killing, while avoiding neutrophil-inflicted tissue damage. We recently showed that the actin-binding protein HS1 promotes neutrophil extravasation; and hypothesized that HS1 is also a critical regulator of sepsis progression. We evaluated the role of HS1 in a model of lethal sepsis induced by cecal-ligation and puncture. We found that septic HS1-deficient mice had a better survival rate compared to WT mice due to absence of lung damage. Lungs of septic HS1-deficient mice showed less inflammation, fibrosis, and vascular congestion. Importantly, systemic CLP-induced neutrophil recruitment was attenuated in the lungs, the peritoneum and the cremaster in the absence of HS1. Lungs of HS1-deficient mice produced significantly more interleukin-10. Compared to WT neutrophils, those HS1-deficient neutrophils that reached the lungs had increased surface levels of Gr-1, ICAM-1, and L-selectin. Interestingly, HS1-deficient neutrophils had similar F-actin content and phagocytic activity, but they failed to polymerize actin and deform in response to CXCL-1 likely explaining the reduced systemic neutrophil recruitment in HS1-deficient mice. Our data show that HS1 deficiency protects against sepsis by attenuating neutrophil recruitment to amounts sufficient to combat bacterial infection, but insufficient to induce tissue damage.
(Copyright © 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.)
Databáze: MEDLINE
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