Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae), isolate HP88, induces reproductive and physiological alterations in Biomphalaria glabrata (Gastropoda: Planorbidae): an alternative for biological control of schistosomiasis.

Autor: Santos Amaral L; Departamento de Medicina Veterinária, Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito Santo (UFES), Alegre, ES, Brazil., Tunholi-Alves VM; Curso de Pós-Graduação em Ciências, Departamento de Parasitologia Animal (DPA), Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rio de Janeiro, Brasil; Universidade Estácio de Sá. Bolsista do Programa Pesquisa e Produtividade da UNESA., Castro LS; Curso de Pós-Graduação em Medicina Veterinária, Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Minas Gerais, Brasil. Electronic address: lorena.scastro@ufv.br., Tunholi VM; Curso de Pós-Graduação em Ciências, Departamento de Parasitologia Animal (DPA), Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rio de Janeiro, Brasil., Gaudêncio F; Centro de Ensino Superior de Valença (CESVA), Rio de Janeiro, Brasil., Monteiro CO; Departamento de Microbiologia, Imunologia, Parasitologia e Patologia da Universidade Federal de Goiás, Rua 235, s/n, Setor Universitário, Goiânia, Goiás, CEP: 74605-050, Brasil., Couto-Chambarelli MCMD; Curso de Pós-Graduação em Ciências, Departamento de Parasitologia Animal (DPA), Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rio de Janeiro, Brasil., Pinheiro J; Curso de Pós-Graduação em Ciências, Departamento de Parasitologia Animal (DPA), Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rio de Janeiro, Brasil; Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, Rio de Janeiro, RJ, Brasil., Freire-Martins IV; Departamento de Medicina Veterinária, Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito Santo (UFES), Alegre, ES, Brazil.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2022 Jun; Vol. 230, pp. 106396. Date of Electronic Publication: 2022 Mar 10.
DOI: 10.1016/j.actatropica.2022.106396
Abstrakt: Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) that is mutually associated with Photorhabdus luminescens, utilized globally for biological control of numerous organisms. Freshwater snails of the species Biomphalaria glabrata have been incriminated as the main intermediate hosts of Schistosoma mansoni in Brazil, but virtually nothing is known about the susceptibility of these gastropod to EPNs. Information in this respect is relevant for control of these intermediate hosts, and thus of the helminthiases they transmit. This paper for the first time reports the susceptibility of B. glabrata to infective juveniles of H. bacteriophora (isolate HP88) under laboratory conditions. For that purpose, six groups were formed: three Control groups (not exposed) and three Treated groups, in which the snails were exposed to 300 juveniles infecting the nematode over three weeks. The entire experiment was conducted in triplicate, using a total of 270 snails. Significant physiological alterations in B. glabrata were observed in response to the infection by H. bacteriophora HP88, characterized by decreased levels of hemolymphatic glucose as well as reduced contents of glycogen stored in the host's digestive gland. In parallel, the hemolymphatic activity of lactate dehydrogenase increased in the infected snails, indicating that the infection induces breakdown of carbohydrate homeostasis in B. glabrata. Additionally, all the reproductive parameters analyzed were reduced as a consequence of the infection. The results indicate the occurrence of the phenomenon of parasitic castration in the B. glabrata/H. bacteriophora HP88 interface, probably due to the depletion of galactogen in the parasitized organism. Although the infection did not cause lethality in the population of infected snails, H. bacteriophora HP88 compromised the reproductive performance of B. glabrata, suggesting its applicability in programs for biological control of this planorbid.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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