Effects of carvacrol and thymol on the antioxidant and detoxifying enzymes of Rhipicephalus microplus (Acari: Ixodidae).

Autor: Tavares CP; Laboratório de Controle de Parasitos, Departamento de Patologia, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil., Sabadin GA; Faculdade de Veterinária and Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil., Sousa IC; Laboratório de Controle de Parasitos, Departamento de Patologia, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil., Gomes MN; Laboratório de Controle de Parasitos, Departamento de Patologia, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil., Soares AMS; Laboratório de Bioquimica Vegetal, Departamento de Engenharia Química, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil., Monteiro CMO; Departamento de Biociências e Tecnologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil., Vaz IS Jr; Faculdade de Veterinária and Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil., Costa-Junior LM; Laboratório de Controle de Parasitos, Departamento de Patologia, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil. Electronic address: livio.martins@ufma.br.
Jazyk: angličtina
Zdroj: Ticks and tick-borne diseases [Ticks Tick Borne Dis] 2022 May; Vol. 13 (3), pp. 101929. Date of Electronic Publication: 2022 Feb 23.
DOI: 10.1016/j.ttbdis.2022.101929
Abstrakt: The present study was conducted to evaluate the effects of carvacrol and thymol on the antioxidant and detoxifying enzymes of larvae from two populations of R. microplus: Jaguar (tick population resistant to six classes of acaricides) and Porto Alegre (susceptible tick population). Carvacrol and thymol were tested at concentrations ranging from 0.14 to 5.0 mg mL -1 in both populations to determine the LC 50 . In addition, the LC 1 , LC 25 , and LC 75 were estimated using the LC 50 and HillSlope of each compound. Larvae of both populations of R. microplus were then treated with the LC 1 , LC 25 , LC 50 , and LC 75 of each monoterpene, and those that survived were processed to evaluate the effects of the compounds on the antioxidant and detoxifying systems of larvae; these effects were assessed by determining the activity of the enzymes, glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX). Larvae from the Jaguar population treated with different lethal concentrations of carvacrol and thymol displayed a dose-dependent increase in CAT, GPX, SOD, and GST after treatment with LC 25 . Further, larvae treated with the LC 75 had the highest levels of enzyme activity for carvacrol (1.76 mg mL -1 ) and thymol (1.32 mg mL -1 ). CAT, GPX, SOD, and GST activity in Porto Alegre population larvae treated with carvacrol and thymol also increased significantly up to the LC 50 of each monoterpene. However, at the LC 75 of carvacrol and thymol, a decrease in the activity of all enzymes was observed for this tick population. These findings indicate that carvacrol and thymol induced increased activity of all evaluated enzymes at different lethal concentrations in R. microplus larvae from two populations. Such findings unveil the possible mechanisms of action of these natural acaricides.
(Copyright © 2022. Published by Elsevier GmbH.)
Databáze: MEDLINE
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