Analysis of HIV-1 integrase genotypes and polymorphisms among integrase inhibitors-based antiretroviral treatment naïve patients in South Sudan.

Autor: Giovanetti M; Reference Laboratory of Filovirus, Oswaldo Cruz Institute, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.; Laboratório de Genética Celular e Molecular, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, Rome, Italy., Farcomeni S; National HIV/AIDS Research Center, Istituto Superiore di Sanità, Rome, Italy., Sernicola L; National HIV/AIDS Research Center, Istituto Superiore di Sanità, Rome, Italy., Virtuoso S; National HIV/AIDS Research Center, Istituto Superiore di Sanità, Rome, Italy., Fontanelli Sulekova L; Department of Translation and Precision Medicine, 'Sapienza' University of Rome, Rome, Italy., Maggiorella MT; National HIV/AIDS Research Center, Istituto Superiore di Sanità, Rome, Italy., Buttò S; National HIV/AIDS Research Center, Istituto Superiore di Sanità, Rome, Italy., Taliani G; Chronic Infectious Diseases Unit, Policlinico Umberto I, 'Sapienza' University of Rome, Rome, Italy., Ciccozzi M; Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, Rome, Italy., Borsetti A; National HIV/AIDS Research Center, Istituto Superiore di Sanità, Rome, Italy.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2022 Jul; Vol. 94 (7), pp. 3320-3327. Date of Electronic Publication: 2022 Mar 26.
DOI: 10.1002/jmv.27713
Abstrakt: HIV-1 genetic diversity and drug resistance mutations remain public health challenges especially in regions where treatment is limited. The aim of this study was to characterize the HIV-1 integrase (IN) subtype and the possible occurrence of drug-resistance mutations or polymorphisms in resource-poor settings in South Sudan. Dried blood spots from integrase inhibitor treatment (Integrase strand transfer inhibitor [INSTI]) naïve HIV-1 infected patients were subjected to DNA amplification and direct sequencing of integrase genes. The sequences were interpreted for drug resistance according to the Stanford algorithm and the International AIDS Society-USA guidelines. Phylogenetic analysis revealed that HIV-1 subtype D, C, G, A1, and recombinant forms accounted for 40%, 10%, 13.3%, 23.4%, and 13.3%, respectively. Furthermore, inter-subtype recombinants were interspersed within viral strains sampled in other African countries, highlighting complex transmission dynamics within a mobile host population. A total of 78 of 288 (27%) amino acid IN positions presented at least one polymorphism each. Major INSTI resistance mutations were absent, however, polymorphic accessory mutations at positions M50ILR (26.6%) and L74I (3.3%) were detected. Despite the limited size of the study population, our findings underscore the need for monitoring minor and natural polymorphisms that may influence the outcome of treatment regimens.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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